Sequences from confirmation amplicon


Confirmation sequences for line 718A03 and hit At1g70480 are shown.
Line ID 718A03
Locus At1g70480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26563529
Primers used for PCR 35cp - 8474, show primer details
   
Sequence Name 49-K108895-0022-718-A03C-35cp
GenBank Accession LN506867 [GenBank]
Sequencing primer gene specific primer, 35cp: 5'-GTGAATGTTGGTTTACCTCCTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K108895-0022-718-A03C-35cp
AAGAGTCTTCAGAATATCAAGGTACGTTTCTTATAGATTATCTTGTTCTCTCTGTTACAG
TTTTAAGCTCCTGTGATTCATTGGCCTCGGAATTACAGGAGGTTATGTCGCGGAAAGTAT
TGGTAGATGACGGGTTAAGGCAAGTGGTGGAAGTTGAACAAGCAGCTTTGTGGAGATTTC
TCTGGTGGTCAGGAACAATCTCAGTTCATGTCTTGGTGGATCAGGATCGGGCAGATCACT
CTGTAAGACCTCTACGATTATAACAAAAGTCTCAAGTTTCTTGAATGGTTCGTGAAAGGG
GATTTAAATGGGTGTTGCAGATGAAATTCAAGCAAGTGAAAAGTGGATTCATGAAGAGAT
TCGAAGGAAGCTGGCAAGTAAAGCCTTTGTTTGTGGATGAACATATGTGCGATCGTTTGA
AACCGAAAACGCTGGAGGAGTATAACAGGTGCACTGGAGGTAAAGGGAGGATCGGATCTA
AGGTAACGCTGGACCAGCTAATACAGCCAGCGATTGTTCCACCTCCGCCCATTTCTTGGT
ATCTCAGAGGAATCACGGCTAAGACGACAGAGATGCTTATCCATGACTTGTTGGCTGAGA
CCGCCAGGATCCGTGCAGCTGGAGTAATGGAGGATGGTCACTCGCCCGATGAGCAACGGA
TTGGATCAGCAATGAGTATTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCG
   
Sequence Name 33-K108894-0022-718-A03C-8409
GenBank Accession LN501029 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K108894-0022-718-A03C-8409
CATTTCAAAATACTCATTGCTGATCCATCCGTTGCTCATCGGGCGAGTGACCATCCTCCA
TTACTCCAGCTGCACGGATCCTGGCGGTCTCAGCCAACAAGTCATGGATAAGCATCTCTG
TCGTCTTAGCCGTGATTCCTCTGAGATACCAAGAAATGGGCGGAGGTGGAACAATCGCTG
GCTGTATTAGCTGGTCCAGCGTTACCTTAGATCCGATCCTCCCTTTACCTCCAGTGCACC
TGTTATACTCCTCCAGCGTTTTCGGTTTCAAACGATCGCACATATGTTCATCCACAAACA
AAGGCTTTACTTGCCAGCTTCCTTCGAATCTCTTCATGAATCCACTTTTCACTTGCTTGA
ATTTCATCTGCAACACCCATTTAAATCCCCTTTCACGAACCATTCAAGAAACTTGAGACT
TTTGTTATAATCGTAGAGGTCTTACAGAGTGATCTGCCCGATCCTGATCCACCAAGACAT
GAACTGAGATTGTTCCTGACCACCAGAGAAATCTCCACAAAGCTGCTTGTTCAACTTCCA
CCACTTGCCTTAACCCGTCATCTACCAATACTTTCCGCGACATAACCTCCTGTAATTCCG
AGGCCAATGAATCACAGGAGCTTAAAACTGTAACAGAGAGAACAAGATAATCTATAAGAA
ACGTACCTTGATATTCTTGAAGACTCTTCTATTGTCAGGGTCAATTACAATGTTATAGAC
TGCATCAGGAGGTA

Last Updated on 06.12.2018 16:11