Sequences from confirmation amplicon


Confirmation sequences for line 718B04 and hit At2g16505 are shown.
Line ID 718B04
Locus At2g16505 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7157913
Primers used for PCR 22wm - 8474, show primer details
   
Sequence Name 58-K118635-0022-718-B04C-22wm
GenBank Accession LN509836 [GenBank]
Sequencing primer gene specific primer, 22wm: 5'-CGATACAACAAGCACAAGTGAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K118635-0022-718-B04C-22wm
GGCATGCAACTGGTTCATTGCCTTGTTCGATTCAACACTTTTTCCATTTTCACCTATACA
AAAAGAAGTAACACAATCATTTGGAAAAATACAGATTATGAAAATATGATTATGTGACTG
TAGAGGTGATGATGATTAAAAGTAAAATAAAATATATATGCATACATTCATGAAGAGGAA
AGAAAGAAATCAGGATGATACAAAGGATTAAAAACCGTGATGAAGACATTGTTGTGATAT
TTTTTACGAAGCAAATACGTTTTGTTTTCTTGATGGTTAAAATTTCTATATCAAGTAAGG
CTTTATATATCTTGAAATATATAATTAGTGGTTTTTGTTATATGGTGATCCATAAAAAAA
GGTAGATTTTGTTTGACTAGTTTTAATTTGTTTTCTTTACTATTACGTGCTAATAGTATG
GTTAACGTTTCCGACGACGAGTAAATGAGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 18-K118634-0022-718-B04C-8409
GenBank Accession LN494521 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118634-0022-718-B04C-8409
ATTACTCATTTACTCGTCGTCGGAAACGTTAACCATACTATTAGCACGTAATAGTAAAGA
AAACAAATTAAAACTAGTCAAACAAAATNCTACCTTTTTTTATGGATCACCATATAACAA
AAACCACTAATTATATATTTCAAGATATATAAAGCCTTACTTGATATAGAAATTTTAACC
ATCAAGAAAACAAAACGTATTTGCTTCGTAAAAAATATCACAACAATGTCTTCATCACGG
TTTTTAATCCTTTGTATCATCCTGATTTCTTTCTTTCCTCTTCATGAATGTATGCATATA
TATTTTATTTTACTTTTAATCATCATCACCTCTACAGTCACATAATCATATTTTCATAAT
CTGTATTTTTCCAAATGATTGTGTTACTTCTTTTTGTATAGGTGAAAATGGAAAAAGTGT
TGAATCGAACAAGGCAATGAAACCAGTTTGCATGCCAGTGAATTGCAACAATAAAGACAA
AAAACTCACTTGTGCT

Last Updated on 06.12.2018 16:11