Sequences from confirmation amplicon


Confirmation sequences for line 718B05 and hit At1g67110 are shown.
Line ID 718B05
Locus At1g67110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25063175
Primers used for PCR 56lm - 8474, show primer details
   
Sequence Name 35-K114022-0022-718-B05C-56lm
GenBank Accession LN501911 [GenBank]
Sequencing primer gene specific primer, 56lm: 5'-AAAGCCAATTCATCATGAGTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114022-0022-718-B05C-56lm
TGTAAATTTCACTTTAGTCTAATAATTAAATACCTGAATTTGTTATATGTCATACATTTG
AAATGAATTATCTGACTTGAAACTACATATCATAATTAATGGGATAACATATTAAGAAGA
TTTTCTAGATATATCATATTAAAGTTGACCGGATATGTACTAATTGAGATTTAGTTACGG
AAGAAGCACTACGACTTAGTGGCATTGCCTGTAAGTGTGTTGAATGAACGTAGTACCCCA
CCCGCACCCATAACAATTGGCGTTACCATCAAATTTTTATTTTATTTTTCAAATGAAAGA
TATAATTGTGATTCTATCCTAATTCTCGACCAAAGTCAAAACTTTACTAGACATGTGTAT
ATGCATAACAACGCANAAGACATTAACACATAGTATGTTTCAATTTTTTAAAATGTTATA
CTATTGTCTTCACTTCTTTTAAAATCTCAGATTTTTTTTATAAGATTCTTATCTTTTGTA
AAACTGTAATGGAAACGTGAAACTCGTATGATCTTTCTCTTTCCGTTTGCTTTTAAAACA
TTCAAAAAGAGTTCGGAGTTTTTGTTCGGAGTTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 14-K114021-0022-718-B05C-8409
GenBank Accession LN492593 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114021-0022-718-B05C-8409
TCCGAACAAAAACTCCGAAACTCTTTTTGAATGTTTTAAAAGCAAACGGAAAGAGAAAGA
TCATACGAGTTTCACGTTTCCATTACAGTTTTACAAAAGATAAGAATCTTATAAAAAAAA
TCTGAGATTTTAAAAGAAGTGAAGACAATAGTATAACATTTTAAAAAATTGAAACATACT
ATGTGTTAATGTCTTATGCGTTGTTATGCATATACACATGTCTAGTAAAGTTTTGACTTT
GGTCGAGAATTAGGATAGAATCACAATTATATCTTTCATTTGAAAAATAAAATAAAAATT
TGATGGTAACGCCAATTGTTATGGGTGCGGGTGGGGTACTACGTTCATTCAACACACTTA
CAGGCAATGCCACTAAGTCGTAGTGCTTCTTCCGTAACTAAATCTCAATTAGTACATATC
CGGTCAACTTTAATATGATATATCTAGAAAATCTTCTTAATATGTTATCCCATTAATTAT
GATATGTAGTTTCAAGTCAGATAATTCATTTCAAATGTATGACATATAACAAATTCAGGT
ATTTAATTATTAGACTAAAGTGAAATTTACATTACCATTGCTTCGACATTTGATTAGATA
GACCAATAGAGAGACTC

Last Updated on 06.12.2018 16:11