Sequences from confirmation amplicon


Confirmation sequences for line 718C03 and hit At2g34180 are shown.
Line ID 718C03
Locus At2g34180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14430976
Primers used for PCR CD22 - 8474, show primer details
   
Sequence Name 21-K029489-022-718-C03C-8409
GenBank Accession LN495539 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K029489-022-718-C03C-8409
CCATTTACAATTGAATACATCCTTCTAGCCTCACGCTCCAATCCTTCTTCCTCACCATAA
ATTTCACCTCTTTGGCAATCTCTTCCAATTTCGATATGATCTTTGTCATAGGAGCAGCAG
ATACAAACCTTGCTCCTTGTCCACCTTCTTCAAACAAACCCGAAAGATCTGAGAACGATA
AGATGTCAAATGCGTTTAGACTCGCGGGTCTCGGCATTGAATCAACCCTTTTAATATCAA
ACTCTGCATCTCCTTCTGAAGCAGTCGATGATCGGCCTGATGACAATGATGATGAATCGT
CATCATCATTGTCATCATCCTCCCTACATAACTTATCGTTTTCAATATAGAATTTGACAT
GTTTGAACCCTTTCTTGAACCATCNTATGCTT
   
Sequence Name 22-K029489-022-718-C03C-CD22
GenBank Accession LN495984 [GenBank]
Sequencing primer gene specific primer, CD22: 5'-GAAGCATAGATGGTTCAAGAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K029489-022-718-C03C-CD22
AGGGTGGATGATGACAATGATGATGACGATTCATCATCATTGTCATCAGGCCGATCATCG
ACTGCTTCAGAAGGAGATGCAGAGTTTGATATTAAAAGGGTTGATTCAATGCCGAGACCC
GCGAGTCTAAACGCATTTGACATCTTATCGTTCTCAGATCTTTCGGGTTTGTTTGAAGAA
GGTGGACAAGGAGCAAGGTTTGTATCTGCTGCTCCTATGACAAAGATCATATCGAAATTG
GAAGAGATTGCCAAAGAGGTGAAATTTATGGTGAGGAAGAAGGATTGGAGCGTGAGGCTA
GAAGGATGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11