Sequences from confirmation amplicon


Confirmation sequences for line 719D11 and hit At5g04060 are shown.
Line ID 719D11
Locus At5g04060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1100207
Primers used for PCR GE48 - 8474, show primer details
   
Sequence Name 94-K100942-0022-719-D11C-8409
GenBank Accession LN515291 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K100942-0022-719-D11C-8409
GCCATTTACAATTGAATATATCCCTGAATTTCTGTATTGTAATACAGGTTAGGAAACATG
ACAACTAATGAAACAGGTGACCTTCTTTCAGCTGGGGTTGAACAAGTTCTTGATGTTGGA
TGTGGAGTTGCTAGCTTTGCAGCTTATCTTCTTCCTTTAGGTATAAAGACAATGTCTTTT
GCCCCTAAAGATGGTCATGAAAACCAAATCCAGTTTGCATTGGAGAGAGGAATCCGCGCA
ATGATCTCTGCCATAGCCACCAAACAAATGCCATATCCCGCGGCCTCGTTTGATATGGTT
CACTGTTCAGATGGTCGTGT
   
Sequence Name 94-K100950-0022-719-D11C-GE48
GenBank Accession LN515292 [GenBank]
Sequencing primer gene specific primer, GE48: 5'-AACACGACATCTTGAACAGTGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K100950-0022-719-D11C-GE48
GCGCGGGATATGGATTTGTTTGGTGGCTATGGCAGAGATCATTGCGCGGATTCCTCTCTC
CATGCAAACTGGATTTGGTTTTCATGACCATCTTTAGGGGCAAAAGACATTGTCTTTATA
CCTAAAGGAAGAAGATAAGCTGCAAAGCTAGCAACTCCACATCCAACATCAAGAACTTGT
TCAACCCCAGCTGAAAGAAGGTCACCTGTTTCATTAGTTGTCATGTTTCCTAACCTGTAT
TACAATACAGAAATTCAGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11