Sequences from confirmation amplicon


Confirmation sequences for line 719G04 and hit At5g45550 are shown.
Line ID 719G04
Locus At5g45550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18462866
Primers used for PCR NH74 - 8474, show primer details
   
Sequence Name 57-K102594-0022-719-G04C-8409
GenBank Accession LN509379 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K102594-0022-719-G04C-8409
GCCATTTACATGGTTGTCGATCAGATTTGTTACATGTATATTGTTATACTAGGCACATTC
AATGGTGGTCGATCAGATTTGTTTTGTTTCTCTGAACTCATGATTGTAGGCAATAAAAAT
GGAATCAAAATACGAGTTTTTAGTGATTCATCCCTGTCATACGCAATGATTTCTGGGATA
CATAGGGTTTACTTATTGATAAAGACTGAAATTTTTATGTTGAACTTATAGCCGTTTTGA
CCCGATCACGTTTACTAACATCTGCTCCTCCTTTTTTATGCTTTCTTAATAGAAATCAGA
AGACTTTCCGACCTAAGAAGAGTGCACCTTCTGGAAGCAAGGTTTGTAAACTATTTGACC
GTCTTCTTCCTATGGTTTATGTCTTTGAAACTACGTATGTAGTTTTGAGTTTTGCAGTC
   
Sequence Name 57-K102595-0022-719-G04C-NH74
GenBank Accession LN509380 [GenBank]
Sequencing primer gene specific primer, NH74: 5'-GTCAACTGATAAACGACTGCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K102595-0022-719-G04C-NH74
AAGACTAAACCATAGGAAGAGACGGTCAATAGTTTACAAACCTTGCTTCCAGAAGGTGCA
CTCTTCTTAGGTCGGAAAGTCTTCTGATTTCTATTAAGAAAGCATAAAAAAGGAGGAGCA
GATGTTAGTAAACGTGATCGGGTCAAAACGGCTATAAGTTCAACATAAAAATTTCAGTCT
TTATCAATAAGTAAACCCTATGTATCCCAGAAATCATTGCGTATGACAGGGATGAATCAC
TAAAAACTCGTATTTTGATTCCATTTTTATTGCCTACAATCATGAGTTCAGAGAAACAAA
ACAAATCTGATCGACCACCATTGAATGTGCCTAGTATAACAATATACATGTAACAAATCT
GATCGACAACCATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11