Sequences from confirmation amplicon


Confirmation sequences for line 720E04 and hit At5g07850 are shown.
Line ID 720E04
Locus At5g07850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2509991
Primers used for PCR NF13 - 8474, show primer details
   
Sequence Name 02-K102427-0022-720-E04C-8409
GenBank Accession LN486977 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K102427-0022-720-E04C-8409
TTACAATTTAATGAAGAACTTACCTTATGATCATGAAATTCGTTGTAGCCTTGCCGCTAA
CAATGGAACAAAATTGGATCCACCATTGTCTTTGAGCTATCTTGGTAATTGTCTTTCTGC
TGTAAAGTCCAAGACCGTTACATCCGGTGAGCTACTAGAAAATGATTTGGGATGGGCTGC
TTTGAAAATGCATGAAGCTGTGATTGGAAACACTAGTGAAGTGGTCTCGGAGACAATCAA
AAACTGGTTGAAATCATCTTATGTTTTCCATTTAGAAAAACTTTTGGGAGCGATGGTGGT
TCACATTGGAAGCTCTCCGAGGTTTAAGATGTATGAATGCGAGTTTGGGATGGGAAAAGC
GGTTGCGGTTAGAAGCGGTTATGGCGGTAAGTTTGATGGGAAGATATCAGCATATGCAGG
AAGAGAAGGAGGAGGAACCATAGATTTGGAAGTATGTCTTCTTCCAGAGTTTATGGAAGC
TTTGGAATCAGATCAAGAATTCATGTCCGTTGTCTCTTCTTCTTCTTGATCTTATTCATA
TAAGTTTGATTGATTGTTTGTGTTGCTGT
   
Sequence Name 02-K102428-0022-720-E04C-NF13
GenBank Accession LN486978 [GenBank]
Sequencing primer gene specific primer, NF13: 5'-TTGGACTTTAACAGCAACACAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K102428-0022-720-E04C-NF13
TAGATCAGAAGAAGAGAGACACGGACATGAATTCTTGATCTGATTCCAAAGCTTCCATAA
ACTCTGGAAGAAGACATACTTCCAAATCTATGGTTCCTCCTCCTTCTCTTCCTGCATATG
CTGATATCTTCCCATCAAACTTACCGCCATAACCGCTTCTAACCGCAACCGCTTTTCCCA
TCCCAAACTCGCATTCATACATCTTAAACCTCGGAGAGCTTCCAATGTGAACCACCATCG
CTCCCAAAAGTTTTTCTAAATGGAAAACATAAGATGATTTCAACCAGTTTTTGATTGTCT
CCGAGACCACTTCACTAGTGTTTCCAATCACAGCTTCATGCATTTTCAAAGCAGCCCATC
CCAAATCATTTTCTAGTAGCTCACCGGATGTAACGGTCTTGGACTTTACAGCAGAAAGAC
AATTACCAAGATAGCTCAAAGACAATGGTGGATCCAATTTTGTTCCATTGTTAGCGGCAA
GGCTACAACGAATTTCATGATCATAAGGTAAGTTTCTTCATTAAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11