Sequences from confirmation amplicon


Confirmation sequences for line 720E06 and hit At3g62020 are shown.
Line ID 720E06
Locus At3g62020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22971502
Primers used for PCR CV15 - 8474, show primer details
   
Sequence Name 13-K030753-022-720-E06C-8409
GenBank Accession LN491913 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K030753-022-720-E06C-8409
CATTTACAATTGAATATATCCTGGGAAAATTACAAATCGGAACGAAAGAAATCGAAAAAA
TCAAATCTAAGTTTGCTCCCAAGAAAGTCTAAAAATCGCGTCGTTATATATATATATTTT
AAATTTTATTAATTGGTTTTTAAATTTTAATAAAGGAAAAAATAAACCTTGTTCATTAGA
ATAACCTGCTGAATCAGCTGGAGGGTTTTAAGCAATGCATATAAAGGTACAAACCTATAA
ACAGAGATCTCTCTACAAAGCAGACGAAGGAGAAGAGAAGGCTGTACAATTTTTTATACA
CTAAAAGCTCTGTATAAACCAAACAACATAACTTTGCCTAGATTTATACATATATGTATG
GACACTGAAACCCA
   
Sequence Name 52-K030753-022-720-E06C-CV15
GenBank Accession LN507706 [GenBank]
Sequencing primer gene specific primer, CV15: 5'-TTTGGGTTTCAGTGTCCATACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K030753-022-720-E06C-CV15
GCAGTATGTTGTTTGGTTTATACGAGCTTTTAGTGTATAAAAAATTGTACAGCCTTCTCT
TCTCCTTCGTCTGCTTTGTAGAGAGATCTCTGTTTATAGGTTTGTACCTTTATATGCATT
GCTTAAAACCCTCCAGCTGATTCAGCAGGTTATTCTAATGAACAAGGTTTATTTTTTCCT
TTATTAAAATTTAAAAACCAATTAATAAAATTTAAAATATATATATATAACGACGCGATT
TTTAGACTTTCTTGGGAGCAAACTTAGATTTGATTTTTTCGATTTCTTTCGTTCCGATTT
GTAATTTTCCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11