Sequences from confirmation amplicon


Confirmation sequences for line 720H07 and hit At2g22770 are shown.
Line ID 720H07
Locus At2g22770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9684701
Primers used for PCR NA48 - 8474, show primer details
   
Sequence Name 05-K101562-0022-720-H07C-8409
GenBank Accession LN488292 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K101562-0022-720-H07C-8409
GCCATTTACATTGAATATATCAAACAAAATCATACATACTCCAGAACAAAGGAAAATTTA
AAATATATCTCCTTATATACTCCTAAAGTTTTCTTTCCTTTAGCTTCTTGTTTAGTTTTA
TTCTTTCAACATTAACTTTTTTTTACTCTCTTGTCAAAAGAGAAAAAGAGTTCTTGTAAT
GGATGATTCAAGCTTTATGGATTTGATGATCGACACTGACGAGTATCTGATCGATGACTG
GGAATCCGATTTCCCGATATGCGGGGAAACTAACACCAACCCCGGTTCCGAGTCTGGATC
TGGAACCGGGTTTGAGTTGCTAGCAGAAAGACCAACGAAGCAAATGAAAACCAACAACAA
CATGAATTCAACATCTTCTTCTCCGTCGTCTTCTTCTTCTTCTGGTTCTCGCACGTCACA
AGTGATCTCATTTGGGTCTCCAGACACGAAAACCAACCCGGTCGAGACATCTTTGAACTT
CTCCAACCAAGTAAGCATGGATCAGAAAGTGGGGTCGAAAAGAAAAGATTGTGTTAACAA
TGGAGGAAGAAGAGAACCACATCTNTNGAAAGAACAGTTTTTTGGCTG
   
Sequence Name 05-K101571-0022-720-H07C-NA48
GenBank Accession LN488293 [GenBank]
Sequencing primer gene specific primer, NA48: 5'-CAGCCAAAACATGTTCTTTCAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K101571-0022-720-H07C-NA48
TCTTTTCTTTTCGACCCCACTTTCTGATCCATGCTTACTTGGTTGGAGAAGTTCAAAGAT
GTCTCGACCGGGTTGGTTTTCGTGTCTGGAGACCCAAATGAGATCACTTGTGACGTGCGA
GAACCAGAAGAAGAAGAAGACGACGGAGAAGAAGATGTTGAATTCATGTTGTTGTTGGTT
TTCATTTGCTTCGTTGGTCTTTCTGCTAGCAACTCAAACCCGGTTCCAGATCCAGACTCG
GAACCGGGGTTGGTGTTAGTTTCCCCGCATATCGGGAAATCGGATTCCCAGTCATCGATC
AGATACTCGTCAGTGTCGATCATCAAATCCATAAAGCTTGAATCATCCATTACAAGAACT
CTTTTTCTCTTTTGACAAGAGAGTAAAAAAAAGTTAATGTTGAAAGAATAAAACTAAACA
AGAAGCTAAAGGAAAGAAAACTTTAGGAGTATATAAGGAGATATATTTTAAATTTTCCTT
TGTTCTGGAGTATGTATGATTTTGTTTTGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11