Sequences from confirmation amplicon


Confirmation sequences for line 721B02 and hit At4g00370 are shown.
Line ID 721B02
Locus At4g00370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:163720
Primers used for PCR 21xw - 8474, show primer details
   
Sequence Name 40-K119676-0022-721-B02C-21xw
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 21xw: 5'-ACATATTACAATCAGGCACGGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K119676-0022-721-B02C-21xw
TTTTTTGTGACAAGTAAAGAGACTCCTCTCTATTGTTGGTCTTTGATCACGCTGCAGGTC
TTGAAGTTCAACCTCACTGAATCCGGGCTACTATGCGTTTTGCCATGGCTAACCATGGCT
GTTTTTGCTAATATTGGAGGTTGGATTGCTGATACTCTTGTGAGCAGAGGTCTTTCAATT
ACAAACGTTCGAAAGGCAAGAAATACACTCTTTCACTTTCTGATCATTTACATGTCTCTA
CTACCTCAAAAGGCCAAACTTGTTATGCTGCTTTCAGTTAAAGGAGTGACAACAAACCCT
TTATACCAATAAACTACTCTCATGAATCTATTATGGCTACTCCAAAGGATACTCACGACT
AATAAACTCTTGATTATTGCAGATTATGCAATCAATTGGTTTTCTAGGCCCTGCCTTCTT
CCTGAGTCAGCTGAGCCATGTCAAAACTCCAGCAATGGCAGTTCTCTGCATGGCATGCAG
TCAGGTATTGGTGTCAATAGATCATATGATTGAAAGAGCCATAGAATTATGGATAAGTTT
GGAACTGTTAAAAACATGTTTGCCGTTTTTTATTTCACACCTGCCGACACAAGATTATTC
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATA
TCCGCAC
   
Sequence Name 16-K119674-0022-721-B02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K119674-0022-721-B02C-8409
TTGAAGAATAATCTTGTGTCGGCAGGTGTGAAATAAAAAACGGCAAACATGGTTTTTAAC
AGTTCCAAACTTATCCATAATTCTATGGCTCTTTCAATCATATGATCTATTGACACCAAT
ACCTGACTGCATGCCATGCAGAGAACTGCCATTGCTGGAGTTTTGACATGGCTCAGCTGA
CTCAGGAAGAAGGCAGGGCCTAGAAAACCAATTGATTGCATAATCTGCAATAATCAAGAG
TTTATTAGTCGTGAGTATCCTTTGGAGTAGCCATAATAGATTCATGAGAGTAGTTTATTG
GTATAAAGGGTTTGTTGTCACTCCTTTAACTGAAAGCAGCATAACAAGTTTGGCCTTTTG
AGGTAGTAGAGACATGTAAATGATCAGAAAGTGAAAGAGTGTATTTCTTGCCTTTCGAAC
GTTTGTAATTGAAAGACCTCTGCTCACAAGAGTATCAGCAATCCAACCTCCAATATTAGC
AAAAACAGCCATGGTTAGCCATGGCAAAACGCATAGTAGCCCGGATTCAGTGAGGTTGAA
CTTCAAGACCTGCAGCGTGATCAAAGACCAACAATAGAGAGGAGTCTCTTTACTTGTTCA
CAAAAAATATTAAACAGTAAGGATATAACTGAAGCTGAACTCCGTGCCTGATGNTAATAT
G

Last Updated on 06.12.2018 16:11