Sequences from confirmation amplicon


Confirmation sequences for line 721C03 and hit At5g20260 are shown.
Line ID 721C03
Locus At5g20260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6836829
Primers used for PCR CR46 - 8474, show primer details
   
Sequence Name 11-K107108-0022-721-C03C-8409
GenBank Accession LN491136 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K107108-0022-721-C03C-8409
ATTGAATATATCCTGTAGTGAGACTAAACCTGAGGTTGCCTTATTAGTGATACTCTGTTT
TTTTTGGGGAATTAATATTGGTATAGAACAATAACGTGAACTGTAGTATAACATTGAAAG
TAGCTACAATGGTAACCAAAGGTTTACAAATAATAAAGACTATTTAAAACTGTTTTGAGA
CTGTTTTTGTTTAATGAATTGTATGAATCACTCTTCATAACTCAACTTGGATCAGATGAA
ATCGTTTGTTCTCTATAGGCATTTTGTCTAACTCAAAGGTGACCAATCCAGAGGAGGAAT
CGTACTCGAATGCAATCTCATTTGACTCAACAACGCATCTCTTAGGCTTCACAGAAGAGT
AAGATCCGAATTTGCCACATCCTTTAACTTCCATTACCACTTTCATCTTCTCGGCTTCAT
ATCTAAGTCCTTCGATAGCTCCTCCCGAATTGTAATTGTTTA
   
Sequence Name 27-K107113-0022-721-C03C-CR46
GenBank Accession LN498410 [GenBank]
Sequencing primer gene specific primer, CR46: 5'-AGTAAACATGTACAATTCGGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K107113-0022-721-C03C-CR46
AGATGAAGTGGTAATGGAAGTTAAAGGATGTGGCAAATTCGGATCTTACTCTTCTGTGAA
GCCTAAGAGATGCGTTGTTGAGTCAAATGAGATTGCATTCGAGTACGATTCCTCCTCTGG
ATTGGTCACCTTTGAGTTAGACAAAATGCCTATAGAGAACAAACGATTTCATCTGATCCA
AGTTGAGTTATGAAGAGTGATTCATACAATTCATTAAACAAAAACAGTCTCAAAACAGTT
TTAAATAGTCTTTATTATTTGTAAACCTTTGGTTACCATTGTAGCTACTTTCAATGTTAT
ACTACAGTTCACGTTATTGTTCTATACCAATATTAATTCCCCAAAAAAAACAGAGTATCA
CTACTAAGGCAACCTCAGGTTTAGTCTCACTACAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11