Sequences from confirmation amplicon


Confirmation sequences for line 721C11 and hit At3g60030 are shown.
Line ID 721C11
Locus At3g60030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22168950
Primers used for PCR PG13 - 8474, show primer details
   
Sequence Name 55-K101746-0022-721-C11C-8409
GenBank Accession LN508763 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101746-0022-721-C11C-8409
TCGGACTGAGCCTTTACATTGAAAATGCTCATTGATTAGAATGAATCTCACAGACCTTAT
GACGTCTATGATAATCCTTAACTTTGCTTAAATCAGCTCCACAGTTGTCTACCTGACAAC
AAATCGCCCGACTCGGAATCCCTCCTCCAAGTTTCGTCTTTTTCACACCATTCCCCTCAA
TATTATTACCACCAAGATTCAGAGTAAGTCTATGAGCATCATCATCTTTCAAGTTATCTT
CTTCCATTGCTACCACAGTAACAGCTCTTCTTTTCTTCTTCTTCTCCATCTCTATCCTCC
TTCTCTCCATGATTTCAACATTTCCTTCATCAGAGCACGTAGAAGAGCTGTTGGATGAGC
CGTGATTCAGCTGTGTAGCAACGAAGAGATCACCATTCCATTTCCAATCATTCAAATCCC
ACTCTACACTCCTTTTACCACTAAACCCGTATTCTAAACTGTGACCTTCTACTTCACCTT
CAATT
   
Sequence Name 55-K101752-0022-721-C11C-PG13
GenBank Accession LN508764 [GenBank]
Sequencing primer gene specific primer, PG13: 5'-ATGGAAGCTAGAATTGAAGGTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101752-0022-721-C11C-PG13
GGTTTGTGGTAAAGGAGTGTAGAGTGGGATTTGAATGATTGGAAATGGAATGGTGATCTC
TTCGTTGCTACACAGCTGAATCACGGCTCATCCAACAGCTCTTCTACGTGCTCTGATGAA
GGAAATGTTGAAATCATGGAGAGAAGGAGGATAGAGATGGAGAAGAAGAAGAAAAGAAGA
GCTGTTACTGTGGTAGCAATGGAAGAAGATAACTTGAAAGATGATGATGCTCATAGACTT
ACTCTGAATCTTGGTGGTAATAATATTGAGGGGAATGGTGTGAAAAAGACGAAACTTGGA
GGAGGGATTCCGAGTCGGGCGATTTGTTGTCAGGTAGACAACTGTGGAGCTGATTTAAGC
AAAGTTAAGGATTATCATAGACGTCATAAGGTCTGTGAGATTCATTCTAATCAATGAGCA
TTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAG
ATGTCC

Last Updated on 06.12.2018 16:11