Sequences from confirmation amplicon


Confirmation sequences for line 722C02 and hit At1g01650 are shown.
Line ID 722C02
Locus At1g01650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:235430
Primers used for PCR JF10 - 8474, show primer details
   
Sequence Name 35-K102175-0022-722-C02C-8409
GenBank Accession LN501776 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K102175-0022-722-C02C-8409
CATTTACAATTGAATATATCCTGGATCCATATTGGTATTCAAGCTTCTTCACTCTTTGAG
AAGTAAAAAAGAAAATCTGATACTGACGTTAAATTTGTTATAGTTTCAGATGGTTCCGCC
GCTTTGGAGAATCATATGTCAAAGTTCCTTTCCTGGGTGCTGTCTCATACTTGACTCTGG
CTATTTGTCCCTTTTGCATAGCATTTGCTGTATTTTGGGCGGTTAAACGTCAATACTCTT
ATGCTTGGATAGGGCAAGATATACTTGTAAGCGCGGGTTTCCATCTCTCTCTCTTAACTG
ACCTTTCTTGAATCCATGTCTTTCTACAAGTATACCTACTGTTAAATTTCCATACAAGGC
TACCATGTACATACCTTCATGCATATGTATAAGTAATTGGTGTTAGGTTGACCTATCTGT
AGTCTTAACAAATAACACAAGTTAACAGCTTACTTTTGGTTTTTCTTGCTGACAGGGAAT
CTCACTGATAATCACGNTTCTTC
   
Sequence Name 35-K102179-0022-722-C02C-JF10
GenBank Accession LN501777 [GenBank]
Sequencing primer gene specific primer, JF10: 5'-TGAAGAACCGTGATTATCAGTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K102179-0022-722-C02C-JF10
GCTGTTAACTTGTGTTATTTGTTAAGACTACAGATAGGTCAACCTAACACCAATTACTTA
TACATATGCATGAAGGTATGTACATGGTAGCCTTGTATGGAAATTTAACAGTAGGTATAC
TTGTAGAAAGACATGGATTCAAGAAAGGTCAGTTAAGAGAGAGAGATGGAAACCCGCGCT
TACAAGTATATCTTGCCCTATCCAAGCATAAGAGTATTGACGTTTAACCGCCCAAAATAC
AGCAAATGCTATGCAAAAGGGACAAATAGCCAGAGTCAAGTATGAGACAGCACCCAGGAA
AGGAACTTTGACATATGATTCTCCAAAGCGGCGGAACCATCTGAAACTATAACAAATTTA
ACGTCAGTATCAGATTTTCTTTTTTACTTCTCAAAGAGTGAAGAAGCTTGAATACCAATA
TGGATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11