Sequences from confirmation amplicon


Confirmation sequences for line 722D05 and hit At4g02075 are shown.
Line ID 722D05
Locus At4g02075 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:914127
Primers used for PCR 23vd - 8474, show primer details
   
Sequence Name 42-K118051-0022-722-D05C-23vd
GenBank Accession LN504629 [GenBank]
Sequencing primer gene specific primer, 23vd: 5'-CAATCTACCATTCAGTTGTGTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K118051-0022-722-D05C-23vd
CGGTTCGGTTGTGTCGTTATGAGATTTCATATTTTATATTTCTATATATATTAGTTTGAC
TTACAGACAAATTGGATATTTTTATATCAAAAATTTACATTAGAATCCTAAAACGACATC
TATTTTGCGGGTCGAATCATATGGACTAATTTTGTGAGAAATAATACTAATGTGTACATT
ATTTTTTTGCAGCTTTCAGTATTTTTGCTGATGAAACATACATTCGATGTGATTTACGGA
ACCGAAGAATATCCATTCTCTGTATTTACGGTAAAAATGCTCTTCCTTTTGATTATCCAA
TCATTAAAATCCTTAATGTTGTTTTGACATGTATATTAATACTGTATGATCTGATGGTAA
AAGAATTGGGTGGATGAAAATGAATGCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 06-K118050-0022-722-D05C-8409
GenBank Accession LN488999 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K118050-0022-722-D05C-8409
CCATTTACATTGAATATATCCTGCATTCATTTTCATCCACCCAATTCTTTTACCATCAGA
TCATACAGTATTAATATACATGTCAAAACAACATTAAGGATTTTAATGATTGGATAATCA
AAAGGAAGAGCATTTTTACCGTAAATACAGAGAATGGATATTCTTCGGTTCCGTAAATCA
CATCGAATGTATGTTTCATCAGCAAAAATACTGAAAGCTGCAAAAAAATAATGTACACAT
TAGTATTATTTCTCACAAAATTAGTCCATATGATTCGACCCGCAAAATAGATGTCGTTTT
AGGATTCTAATGTAAATTTTTGATATAAAAATATCCAATTTGTCTGTAAGTCAAACTAAT
ATATATAGAAATATAAAATATGAAATCTCATAACGACACAACCGAACCGCATCATATCAC
ATATGTAAACAATATCCCTCGAGATTGGCTCGTTTGAACTTTAATTAATTTCGGATGAGA
AAAAATAAAGAACGACACAACTGATGGGGTAGATTG

Last Updated on 06.12.2018 16:11