Sequences from confirmation amplicon


Confirmation sequences for line 723A03 and hit At1g04410 are shown.
Line ID 723A03
Locus At1g04410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1189745
Primers used for PCR GE61 - 8474, show primer details
   
Sequence Name 10-K100994-0022-723-A03C-8409
GenBank Accession LN490577 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K100994-0022-723-A03C-8409
TCGGAATGAGCCTTTACATTGAATATATCCTGTTGACCGACATGTAGATTAACTGTTACA
AAAAAACAGCACTGCAAATAACGGGGAAGAGTTTTATTATGAACCTCTGGAGTTCCAAGG
ACCCAGTCACGGATGTGGTCACAAGCAGAGCTAGCAGCAGAGAGCGCACTAGACAACTTC
CTCGCCTTGATGATTGCAGCTCCACGTTGTTGAACTGTAGAGATAAATTCTCCGTCCAAC
CTACAGAATGAGCAATATATTTAAGTGAAACTAGATTTTGCTTCCAGAGAAGAGAAAGGG
TCTTAAGTTCTATGGCATGTTATACATACCATGCATCGTCCTTGACGAGCTCACGGACAG
GCTTCTCTCCAGACGAGGTCTGCACTTTAGCATGGTTGACATCTGGGTACTGTGAGGATG
AGTGGTTTCCCCAGATGATCACGTTCTTAACATCAGACACTGGCACGCTCAACCTCTCAG
AGATCTGTCCCAAAGCCCTGTTGTGGTCAAGCCTTGTCAAACAAGAGATGTTC
   
Sequence Name 10-K100998-0022-723-A03C-GE61
GenBank Accession LN490578 [GenBank]
Sequencing primer gene specific primer, GE61: 5'-TCAATCCCTGAAAAGAACATCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K100998-0022-723-A03C-GE61
GCTTTGGGACAGATCTCTGAGAGGTTGAGCGTGCCAGTGTCTGATGTTAAGAACGTGATC
ATCTGGGGAAACCACTCATCCTCACAGTACCCAGATGTCAACCATGCTAAAGTGCAGACC
TCGTCTGGAGAGAAGCCTGTCCGTGAGCTCGTCAAGGACGATGCATGGTATGTATAACAT
GCCATAGAACTTAAGACCCTTTCTCTTCTCTGGAAGCAAAATCTAGTTTCACTTAAATAT
ATTGCTCATTCTGTAGGTTGGACGGAGAATTTATCTCTACAGTTCAACAACGTGGAGCTG
CAATCATCAAGGCGAGGAAGTTGTCTAGTGCGCTCTCTGCTGCTAGCTCTGCTTGTGACC
ACATCCGTGACTGGGTCCTTGGAACTCCAGAGGTTCATAATAAAACTCTTCCCCGTTATT
TGCAGTGCTGTTTTTTTGTAACAGTTAATCTACATGTCGGTCAACAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11