Sequences from confirmation amplicon


Confirmation sequences for line 723E01 and hit At2g19810 are shown.
Line ID 723E01
Locus At2g19810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8551115
Primers used for PCR 17zg - 8474, show primer details
   
Sequence Name 40-K125614-0022-723-E01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K125614-0022-723-E01C-8409
TGAATATATCCTGTAACGACTCAGTAACGAACACGACGAGTCCGAGTCACCTCGTGGACT
CACCGGCGGCGAGTTAGACGACGGAGAAATCGAAAACTGAAACGCTCTACGAATCGTAGG
AGACAAAACGTCGAACGAATCAACACGATCAGGGCTTTGATTAGGCAAAACCCTAATCTG
ATCCGGCGAATGAGCAAAGAAACAAACACGACGGCGACAGTTACCACCGTCTTTACACGG
CTGAGTCCGGTAACGCGCCGGGTGAAGCCAACACTCAAAAACACCATGAGAAAACTCACA
TGCGTCTCCTCTCTTGCAACAACCTTTACGAAACTCAGGACACGCCGTACCGGAGTAATG
AAACTTCCTCGGATCACGACGGCGAGCTTTTTCTCCGGGATGAGCGTAAGGACACTCCGT
CCAATCATGGCTCCGGCCACGAGCACAACGTCTCACTTTAAACTCATACATCCGAAAATG
ATCGCATGTATAAGCATCGATTGGTGAATCCGGACCCGAGAGATCCGGATCTGAATCCGG
ATCCGGTTCGTTTGACGGAAGATAACGTTGTAACTCAGCCAAAGCTTCCATCATACTACT
TCCTCCGTCTGGACTTCCGTAAATATCAGACACCGTTAGATCTTCAGAAAGTGGCCATGG
AGGAATATGAACCGTTGGATTAAAACCACGATGAGATTCTCCGATCATCATTTTTTTACA
ACAATTCACTCAAGAAGAAAAAATCACATTCGTCTGAGTTGGTTTTGTTTTTTGTGTTTG
TGTTTGCTAAATCTCCGGCAAAGCTTTACTTCTTTATAAACTGATGCTGAGTAGTGGGAT
ATACTTAAACCACCCTTTGGGTTAAAACCTAACCATGGTTAAATATTTCTGCTTTTATCA
ACCGCGGTTAAAATCGAAAGTGAAAAGCTACAGTGACGTGTGTGTGAT
   
Sequence Name 17-K125613-0022-723-E01C-17zg
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 17zg: 5'-AGTAATATAACAGCCGCTCACTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K125613-0022-723-E01C-17zg
AATCATCACGNTCATTAATATCCTTGTGGAAAGGTCTCAAGCCGCTGTCCTTAACCTCTG
CATCACACACACGTCACTGTAGCTTCTCACTTTCGATTTTAACCGCGGTTGATAAAAGCA
GAAATATTTAACCATGGTTAGGTTTTAACCCAAAGGTGGTTTAAGTATATCCCACTACTC
AGCATCAGTTTATAAAGAAGTAAAGCTTTGCCGGAGATTTAGCAAACACAAACACAAAAA
ACAAAACCAACTCAGACGAATGTGATTTTTTCTTCTTGAGTGAATTGTTGTAAAAAAATG
ATGATCGGAGAATCTCATCGTGGTTTTAATCCAACGGTTCATATTCCTCCATGGCCACTT
TCTGAAGATCTAACGGTGTCTGATATTTACGGAAGTCCAGACGGAGGAAGTAGTATGATG
GAAGCTTTGGCTGAGTTACAACGTTATCTTCCGTCAAACGAACCGGATCCGGATTCAGAT
CCGGATCTCTCGGGTCCGGATTCACCAATCGATGCTTATACATGCGATCATTTTCGGATG
TATGAGTTTAAAGTGAGACGTTGTGCTCGTGGCCGGAGCCATGATTGGACGGAGTGTCCT
TACGCTCATCCCGGAGAAAAAGCTCGCCGTCGTGATCCGAGGAAGTTTCATTACTCCGGT
ACGGCGTGTCCTGAGTTTCGTAAAGGTTGTTGCAAGAGAGGAGACGCATGTGAGTTTTCT
CATGGTGTTTTTGAGTGTTGGCTTCACCCGGCGCGTTACCGGACTCAGCCGTGTAAAGAC
GGTGGTAACTGTCGCCGTCGTGTTTGTTTCTTTGCTCATTCGCCGGATCAGATTAGGGTT
TTGCCTAATCAAAGCCCTGATCGTGTTGATTCGTTCGACGTTTTGTCTCCAACGATTCGT

Last Updated on 06.12.2018 16:11