Sequences from confirmation amplicon


Confirmation sequences for line 723E02 and hit At4g35090 are shown.
Line ID 723E02
Locus At4g35090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16702268
Primers used for PCR 05ju - 8474, show primer details
   
Sequence Name 67-K113261-0022-723-E02C-05ju
GenBank Accession LN511852 [GenBank]
Sequencing primer gene specific primer, 05ju: 5'-TGTTCTCCTCAATGTTATTGTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113261-0022-723-E02C-05ju
TCTATCCTTCTTGAGATACCATCTCGTTGAGAAGCTTGCCAATTTCGACAGGGAACGGAT
TCCAGAGCGTGTGGTTCATGCCAGAGGAGCCAGTGCTAAAGGTTTCTTTGAGGTCACTCA
TGATATCTCTAACCTCACTTGTGCTGACTTTCTCCGAGCTCCCGGTGTTCAGACTCCTGT
CATTGTCCGGTTCTCCACCGTTATCCATGAGCGTGGAAGTCCCGAGACCTTGAGAGACCC
TCGTGGTTTTGCAGTCAAGTTCTACACCAGAGAGGTCAGTTAGTAGTTTGTAACATTTAA
GAATGCTAGCAATTACTTATATACTAAGCAAATGATCATTCTTTGTATGCAGGGGAACTT
TGATCTTGTTGGAAACAACTTTCCTGTTTTCTTCATCCGCGATGGGATGAAGTTCCCTGA
CATGGTCCACGCTCTTAAGCCGAACCCAAAATCTCACATCCAAGAGAACTGGAGAATCCT
TGACTTCTTCTCCCACAAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 48-K113260-0022-723-E02C-8409
GenBank Accession LN506553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K113260-0022-723-E02C-8409
ATTTACATTGAATATATCCTGTTTGTGGGAGAAGAAGTCAAGGATTCTCCAGTTCTCTTG
GATGTGAGATTTTGGGTTCGGCTTAAGAGCGTGGACCATGTCAGGGAACTTCATCCCATC
GCGGATGAAGAAAACAGGAAAGTTGTTTCCAACAAGATCAAAGTTCCCCTGCATACAAAG
AATGATCATTTGCTTAGTATATAAGTAATTGCTAGCATTCTTAAATGTTACAAACTACTA
ACTGACCTCTCTGGTGTAGAACTTGACTGCAAAACCACGAGGGTCTCTCAAGGTCTCGGG
ACTTCCACGCTCATGGATAACGGTGGAGAACCGGACAATGACAGGAGTCTGAACACCGGG
AGCTCGGAGAAAGTCAGCACAAGTGAGGTTAGAGATATCATGAGTGACCTCAAAGAAACC
TTTAGCACTGGCTCCTCTGGCATGAACCACACGCTCTGGAATCCGTTCCCTGTCGAAATT
GGCAAGCTTCTCAACGAGATGGTAATCCTCAAGAAGGATAGGACCTGCCCAAAAAAACAA
TCAGAAATGCAACAATAACATTGGGGGAGAAC

Last Updated on 06.12.2018 16:11