Sequences from confirmation amplicon


Confirmation sequences for line 723F03 and hit At2g38070 are shown.
Line ID 723F03
Locus At2g38070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15930187
Primers used for PCR 24vd - 8474, show primer details
   
Sequence Name 28-K117967-0022-723-F03C-24vd
GenBank Accession LN499031 [GenBank]
Sequencing primer gene specific primer, 24vd: 5'-GATGACCCTCGTTACTCTTTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117967-0022-723-F03C-24vd
TTTGATTGGAGAGCTGCTGCTCCGATGCGAATGCCGTCGATGTTATCTGTAGTGGAAGAT
TCTCCGGTTCGGAATCATGTTCACCGGTCAGATACTCACATTCCGGTGGAAAAGTCACCA
CAAGTATCAGAAGCTGTGATTGATGAGATTGTTCCTGGTGGATCTGCACAGACCAGAGAG
TATTACTTAGACTCATCATCTTCACGAAGGAGGAAGAGCCTTGACCGTTCTAGCTCCACT
AGGAAGTTATCAGCCTCTGTCATGGCTGAAATTGATGAACTAAAGCTGACTCAAGACAGA
GAGGCTAAAGACTTGGTTTCACATTCGAATTCACTGAGAGACGATTGTTGCTCTGTTGAG
AACAACTATGAGATGGGTGTTAGAGAAAATGTAGGGACTATAGAATGCAACAACAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCC
   
Sequence Name 12-K117966-0022-723-F03C-8409
GenBank Accession LN491779 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117966-0022-723-F03C-8409
ATTTACAATTGAATATATCCTGTTGTTGCATTCTATAGTCCCTACATTTTCTCTAACACC
CATCTCATAGTTGTTCTCAACAGAGCAACAATCGTCTCTCAGTGAATTCGAATGTGAAAC
CAAGTCTTTAGCCTCTCTGTCTTGAGTCAGCTTTAGTTCATCAATTTCAGCCATGACAGA
GGCTGATAACTTCCTAGTGGAGCTAGAACGGTCAAGGCTCTTCCTCCTTCGTGAAGATGA
TGAGTCTAAGTAATACTCTCTGGTCTGTGCAGATCCACCAGGAACAATCTCATCAATCAC
AGCTTCTGATACTTGTGGTGACTTTTCCACCGGAATGTGAGTATCTGACCGGTGAACATG
ATTCCGAACCGGAGAATCTTCCACTACAGATAACATCGACGGCATTCGCATCGGAGCAGC
AGCTCTTCCAATCAAATATCCATCCCACGAAGCGCGTGGCTCCTCAAAAGAGTAA

Last Updated on 06.12.2018 16:11