Sequences from confirmation amplicon


Confirmation sequences for line 723G09 and hit At1g13360 are shown.
Line ID 723G09
Locus At1g13360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4577789
Primers used for PCR 21uv - 8474, show primer details
   
Sequence Name 65-K117671-0022-723-G09C-21uv
GenBank Accession LN511480 [GenBank]
Sequencing primer gene specific primer, 21uv: 5'-ATCTAAACCACCGTAATTCGACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K117671-0022-723-G09C-21uv
CGAATCAGAAGACGCTCGNTACCAAATCCGTTACCGTCTCCGTCGTTACCTCCTCCTTCG
CGACGGGAACCGGAGAAATCGATGGAGGTGGTGGTAAACCAAGCTCATCATCGGAAGCTT
CAAGAAGATATCCGAGATCAGGCTGAGTTTCGCCGGCGGTAGAGGAACCTTGCGCCGTCG
TGGTGGTGACCGTTGATAACTCGTCTTCGAAACTTTTCATAACCGAGTCGAGATCTTGAC
TCACTGGTTCAGGATCCGAGTCATCCAGGACATCGAATAAATCATCTCTCAACCTCTTCA
CCTCCGGCGAGTCAAGAACCGCTTCGTCGAATGACTCGTCTCTGACTCGCTTTTTCTCAG
CCGAGTCAGTCCGAGTTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 17-K117670-0022-723-G09C-8409
GenBank Accession LN494040 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117670-0022-723-G09C-8409
GCCATTTATACTCGGACTGACTCGGCTGAGAAAAAGCGAGTCAGAGACGAGTCATTCGAC
GAAGCGGTTCTTGACTCGCCGGAGGTGAAGAGGTTGAGAGATGATTTATTCGATGTCCTG
GATGACTCGGATCCTGAACCAGTGAGTCAAGATCTCGACTCGGTTATGAAAAGTTTCGAA
GACGAGTTATCAACGGTCACCACCACGACGGCGCAAGGTTCCTCTACCGCCGGCGAAACT
CAGCCTGATCTCGGATATCTTCTTGAAGCTTCCGATGATGAGCTTGGTTTACCACCACCT
CCATCGATTTCTCCGGTTCCCGTCGCGAAGGAGGAGGTAACGACGGAGACGGTAACGGAT
TTGGTACGAGCGTCTTCTGATTCGTCAGGAATCGACGAGATTTGGGGATTTGAAGATCAC
GTGTCGAATTACGGTGGTTTTAGAT

Last Updated on 06.12.2018 16:11