Sequences from confirmation amplicon


Confirmation sequences for line 723H09 and hit At1g44170 are shown.
Line ID 723H09
Locus At1g44170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16798687
Primers used for PCR 47kh - 8474, show primer details
   
Sequence Name 40-K113531-0022-723-H09C-47kh
GenBank Accession LN503772 [GenBank]
Sequencing primer gene specific primer, 47kh: 5'-ATGCCAATCTATCATTTGAAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K113531-0022-723-H09C-47kh
TATTTGCGGCTGATAGAATTTGCTTAGTGATGATATTTCTTTAATTAGTGGCTGTTTACA
CATAAGCCAAACCTCTATAATTGGATTAACATATCCCCCTACTTGGTCGGTCTCATTATT
GTCTATGACCTTAGTTGCTACGTAAATGATGTAGTTTGTCGACACATGTAACCAACACTG
TCTTCAATGCTAACTTTTTCACAAGTTAATACTAGTATCCATCAGCATAGAATGCAGGTA
ACTTTATGTTATGTAGGAACCAATACTATAAAAATGTTGGGTCCTATATTGAAAGCAAAT
ATACATTAAATTTTTCCTTCTCCTACTGTTAAACTACCATATAACATTAAATTTCATCAA
TGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATG
   
Sequence Name 15-K113530-0022-723-H09C-8409
GenBank Accession LN493052 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K113530-0022-723-H09C-8409
ATCCTGCATTGATGAAATTTAATGTTATATGGTAGTTTAACAGTAGGAGAAGGAAAAATT
TAATGTATATTTGCTTTCAATATAGGACCCAACATTTTTATAGTATTGGTTCCTACATAA
CATAAAGTTACCTGCATTCTATGCTGATGGATACTAGTATTAACTTGTGAAAAAGTTAGC
ATTGAAGACAGTGTTGGTTACATGTGTCGACAAACTACATCATTTACGTAGCAACTAAGG
TCATAGACAATAATGAGACCGACCAAGTAGGGGGATATGTTAATCCAATTATAGAGGTTT
GGCTTATGTGTTAAACAGCCACTAATTAAAGAAATATCATCACTAAGCAAATTCTATCAG
CCGCAAATATTAGTAGCGACATCTGATTCTGACTCATAACCATACCAATAATTGGTTTTC
AAATGATAGATTGGCA

Last Updated on 06.12.2018 16:11