Sequences from confirmation amplicon


Confirmation sequences for line 724E06 and hit At4g38960 are shown.
Line ID 724E06
Locus At4g38960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18161249
Primers used for PCR IF41 - 8474, show primer details
   
Sequence Name 80-K101386-0022-724-E06C-8409
GenBank Accession LN513926 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K101386-0022-724-E06C-8409
TAGATACTCTCGGCGCGTGGATTTGGTCGTCGTCTCTTCTGGTCTCTCAAGCTTCTCCTC
CATCGTAGAAAAAGATTTTAAAGGAGCGATTTTTATAATCTTAAATAATTTCTTCCCTTT
CTTCTTCTTCTTCTAGTTCCTTGATACCAACGCGTAATCCTAACCCTTAATCTACTTCCT
CCTTAATTAATCAGAGGTTTCTACTTATTATTTCCACTTCTCATCTCTTTCAATCGCAAT
CTGAGTTCTGGCTAAAGATTTTGTCTTGAAGGTGAGATTCATTGTTCCGAATAGAGTGAG
AAAAAGACTTATAAGATTGAAAAAGCAAGATGCGGATTTTGTGCGATGCTTGCGAGAACG
CAGCCGCAATCATCTTTTGCGCCGCCGATGAAGCTGCCCTTTGTCGCCCCTGCGATGAAN
AAGTAACTTCTCTCCCAAAAAAGCTTCCTTTTTTTCTTTAACTCTCCTCTCTATCTATTG
GTTGTGCTCTGATGGGTTCGTCAAAGATCAAGTCTTTTGTATCTCTGTCTGGGAAATCTC
AGTTGATTTGGACCTGAGTTGATCTGGCTTTTCGATNACTTTGAGCTTAAGAAATAAGAT
TTTTGAGATTTGGTTTCT
   
Sequence Name 80-K101392-0022-724-E06C-IF41
GenBank Accession LN513927 [GenBank]
Sequencing primer gene specific primer, IF41: 5'-ACCAAATGAGAGAAGAGGTTTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K101392-0022-724-E06C-IF41
ACTTCGAAATATCTAATCTCATGTGAAGAGCCTAAGAATCTAGAAACCAAATCTCAAAAA
TCTTATTTCTTTAGCTCAAAGTTATCGAAAAGCCAGATCAACTCAGGTCCAAATCAACTG
AGAATTCCCAGACAGAGATAACAAAGACTTGATCTTTGACGAACCCATCAGAGCACAACC
AATAGATAGAGAGGAGAGTTAAAGAAAAAAAGGAAGCTTTTTTGGGAGAGAAGTTACTTT
TTCATCGCAGGGGCGACAAAGGGCAGCTTCATCGGCGGCGCAAAAGATGATTGCGGCTGC
GTTCTCGCAAGCATCGCACAAAATCCGCATCTTGCTTTTTCAATCTTATAAGTCTTTTTC
TCACTCTATTCGGAACAATGAATCTCACCTTCAAGACAAAATCTTTAGCCAGAACTCAGA
TTGCGATTGAAAGAGATGAGAAGTGGAAATAATAAGTAGAAACCTCTGATTAATTAAGGA
GGAAGTAGATTAAGGGTTAGGATTACGCGTTGGTATCAAGGAACTAGAAGAAGAAGAAGA
AGGGGAAGAATTATTAAGAATATAAAATCGCTCCTTTTAAATCTTTTCTACGATGGAGGA
GAAAGCTGAGAGACCAGAAGAGACGA

Last Updated on 06.12.2018 16:11