Sequences from confirmation amplicon


Confirmation sequences for line 725A05 and hit At4g24770 are shown.
Line ID 725A05
Locus At4g24770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12767058
Primers used for PCR AO52 - 8474, show primer details
   
Sequence Name 38-K105775-0022-725-A05C-AO52
GenBank Accession LN502982 [GenBank]
Sequencing primer gene specific primer, AO52: 5'-CCAAAAACAATTCCACACATAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K105775-0022-725-A05C-AO52
ATAGAAACGAGAAAGTGTGACAATAGTAACATAGAAAGTTTTGGATAACAGCATTCCATA
GACAATAGAAAACTGTTTTCTTAGAGCAGAAAGGACGAAATCAAAGGAGCAATATCCCAT
CACAATACATGCTAATAACTCAGAAACAGAAATGATTGAAAGTTTGAACCATATAAACTT
ACATAACGGTTGAACTTCTCCACAGCTGTCTCAGCTTCGTCCACAGAACTCATTGTCACA
AATCCACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 45-K105776-0022-725-A05C-8409
GenBank Accession LN505535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K105776-0022-725-A05C-8409
TTCGGACATGAGCCATTTACATTGAATATATCCTGTGGATTTGTGACAATGAGTTCTGTG
GACGAAGCTGAGACAGCTGTGGAGAAGTTCAACCGTTATGTAAGTTTATATGGTTCAAAC
TTTCAATCATTTCTGTTTCTGAGTTATTAGCATGTATTGTGATGGGATATTGCTCCTTTG
ATTTCGTCCTTTCTGCTCTAAGAAAACAGTTTTCTATTGTCTATGGAATGCTGTTATCCA
AAACTTTCTATGTTACTATTTGTCACACTTTCTCTGTTTCTATTTGTCAATCGAATATGG
AAGCTTTGGCATTTTATGTGTGGAATTGTTTTTGG

Last Updated on 06.12.2018 16:11