Sequences from confirmation amplicon


Confirmation sequences for line 725H11 and hit At3g15480 are shown.
Line ID 725H11
Locus At3g15480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5227301
Primers used for PCR KH19 - 8474, show primer details
   
Sequence Name 15-K103100-0022-725-H11C-KH19
GenBank Accession LN492930 [GenBank]
Sequencing primer gene specific primer, KH19: 5'-AACGACTAGCAGCCATAATAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103100-0022-725-H11C-KH19
CAGCTCCTAACTTGTAGCAATGTCAGTACCATACACACAGTAATCATATTGCTTGTCTCT
GTCTGTTTCAACCTTGCCCTGTCAAAACAACGACTCCTTCAAGATATGAATCGTGTAAAG
AAAAACATAAGCAGAAACCACATTTCAGATACATACAAGATTTGGATTTGATCTAAACAT
ATAACTTCTTCTTTAGCTAACCACTTGTTCCAATGTGGATATTCACAACACTTATCTAGA
ATGTGGAATTTCAACAATACAGTATTACTAAGAAATTTCTATACTCACTACAATGTTATG
CTCTGCATAAATTCTAGGTCATGAATCGAAACTATGCATTTGGATCTTCAAATTCAAGTG
GAATCAATTGTATATCCATATTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATATCCGCACGGTTTATT
   
Sequence Name 15-K103101-0022-725-H11C-8409
GenBank Accession LN492931 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103101-0022-725-H11C-8409
GCCATTTACATTGAATAATATGGATATACATTGATTCCACTTGAATTTGAAGATCCAAAT
GCATAGTTTCGATTCATGACCTAGAATTTATGCAGAGCATAACATTGTAGTGAGTATAGA
AATTTCTTAGTAATACTGTATTGTTGAAATTCCACATTCTAGATAAGTGTTGTGAATATC
CACATTGGAACAAGTGGTTAGCTAAAGAAGAAGTTATATGTTTAGATCAAATCCAAATCT
TGTATGTATCTGAAATGTGGTTTCTGCTTATGTTTTTCTTTACACGATTCATATCTTGAA
GGAGTCGTTGTTTTGACAGGGCAAGGTTGAAACAGACAGAGACAAGCAATATGATTACTG
TGTGTATGGTACTGACATTGCTACAAGTTATGGAGCTGGTGCATTTGTTCTTCTCTTTGT
AAGCCAAGTCCTTATTATGGCTGCTAGTCGT

Last Updated on 06.12.2018 16:11