Sequences from confirmation amplicon


Confirmation sequences for line 726A06 and hit At3g12110 are shown.
Line ID 726A06
Locus At3g12110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3858474
Primers used for PCR 13hq - 8474, show primer details
   
Sequence Name 35-K112562-0022-726-A06C-13hq
GenBank Accession LN501887 [GenBank]
Sequencing primer gene specific primer, 13hq: 5'-TGAGGTAGTGAATGAGTAACCACG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K112562-0022-726-A06C-13hq
GTCACGGCCTGCAGGGTCCAACGCAGAATAGCATGTGGAAGAGCATATCCTTCATAAATT
GGAACAGTGTGACTCACACCATCTCCAGAGTCCAACACAATACCTGGAAACACACAAGAC
AATGGACAATGGTACCACATCAACAAAGCCAAGAAAGGGTACTTTCTTAATCTGAAGCTA
AGAAGAACTTGGAAGTTTGTTCAATGCAATGGGTAGTAGTGATTAAAAAAATGGTGTAGG
TGTTGAAGATCTTATTACCAGTAGTACGACCACTAGCGTAGAGGGAAAGAACAGCTTGGA
TAGCGACATACATGGCAGGAGTGTTGAAAGTTTCAAACATAATCTGAGTCATTTTCTCAC
GATTGGCCTTGGGGTTGAGAGGAGCCTCAGTGAGAAGAACAGGGTGTTCTTCAGGTGCAA
CACGGAGCTCATTGTAGAAAGTGTGATGCCAAATCTTCTCCATATCTTCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
G
   
Sequence Name 11-K112561-0022-726-A06C-8409
GenBank Accession LN491184 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K112561-0022-726-A06C-8409
TTGAATATATCCTGAAGATATGGAGAAGATTTGGCATCACACTTTCTACAATGAGCTCCG
TGTTGCACCTGAAGAACACCCTGTTCTTCTCACTGAGGCTCCTCTCAACCCCAAGGCCAA
TCGTGAGAAAATGACTCAGATTATGTTTGAAACTTTCAACACTCCTGCCATGTATGTCGC
TATCCAAGCTGTTCTTTCCCTCTACGCTAGTGGTCGTACTACTGGTAATAAGATCTTCAA
CACCTACACCATTTTTTTAATCACTACTACCCATTGCATTGAACAAACTTCCAAGTTCTT
CTTAGCTTCAGATTAAGAAAGTACCCTTTCTTGGCTTTGTTGATGTGGTACCATTGTCCA
TTGTCTTGTGTGTTTCCAGGTATTGTGTTGGACTCTGGAGATGGTGTGAGTCACACTGTT
CCAATTTATGAAGGATATGCTCTTCCACATGCTATTCTGCGTTTGGACCTTGCAGGCCGT
GACCTTACTGATTACCTCATGAAGATCTTAACCGAGCGTGGTTACTCATCACNTACCTC

Last Updated on 06.12.2018 16:11