Sequences from confirmation amplicon


Confirmation sequences for line 726D09 and hit At1g07920 are shown.
Line ID 726D09
Locus At1g07920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2455896
Primers used for PCR 05ip - 8474, show primer details
   
Sequence Name 25-K112877-0022-726-D09C-8409
GenBank Accession LN497524 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K112877-0022-726-D09C-8409
ATCCTTATCATTGACTCCACCACTGGNTGGTTTTGAGGCTGGTATCTCCAAGGATGGTCA
GACCCGTGAGCACGCTCTACTTGCTTTCACCCTTGGTGTCAAGCAGATGATCTGCTGTTG
TAACAAGGTAACCATATCTTTCATCTGTTATGTGACTACACATTGCTTCTCTTTTTGTGT
TCTGTCTCATTAATTGCGGTTTGTTACATGTTGTTTGTAGATGGATGCCACTACCCCCAA
GTACTCCAAGGCCAGGTACGATGAAATCATCAAGGAGGTGTCTTCCTACTTGAAGAAGGT
TGGTTACAACCCCGACAAAATCCCATTTGTGCCCATCTCTGGATTTGAGGGTGACAACAT
GATTGAGAGGTCCACCAACCTTGACTGGTACAAGGGACCAACTCTCCTTGAGGCTCTTGA
CCAGATCAACGAGCCCAAGAGGCCGTCAGACAAGCCCCTTCGTCTCCCACTTCAGGATGT
CTACAAGATTGGTG
   
Sequence Name 62-K112878-0022-726-D09C-05ip
GenBank Accession LN510798 [GenBank]
Sequencing primer gene specific primer, 05ip: 5'-CACCAATCTTGTAGACATCCTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K112878-0022-726-D09C-05ip
GGCTGTTGATCTGGTCAGAGCCTCAAGGAGAGTTGGTCCCTTGTACCAGTCAAGGTTGGT
GGACCTCTCAATCATGTTGTCACCCTCAAATCCAGAGATGGGCACAAATGGGATTTTGTC
GGGGTTGTAACCAACCTTCTTCAAGTAGGAAGACACCTCCTTGATGATTTCATCGTACCT
GGCCTTGGAGTACTTGGGGGTAGTGGCATCCATCTACAAACAACATGTAACAAACCGCAA
TTAATGAGACAGAACACAAAAAGAGAAGCAATGTGTAGTCACATAACAGATGAAAGATAT
GGTTACCTTGTTACAACAGCAGATCATCTGCTTGACACCAAGGGTGAAAGCAAGTAGAGC
GTGCTCACGGGTCTGACCATCCTTGGAGATACCAGCCTCAAAACCACCAGTGGTGGAGTC
AATGATAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCACGGTTATT
Primers used for PCR 12lp - 8474, show primer details
   
Sequence Name 58-K113988-0022-726-D09C-12lp
GenBank Accession LN509767 [GenBank]
Sequencing primer gene specific primer, 12lp: 5'-AAACAAGAACACAGACACG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K113988-0022-726-D09C-12lp
CCTTTGATTCACCTGCGGCAGATAGAGTTTTGAGGATGGTTCACTTCGCACCCTTCTTGA
CGGCAGCCTTGGTAACCTTGGCTCCGGTTGGGTCCTTCTTGTCAACACTCTTGATAACAC
CGACTGCAACAGTCTGCCTCATGTCCCTCACAGCGAAACGTCCAAGTGGTGGGTACTCAG
AGAAGGTCTCAACAACCATGGGCTTGGTTGGAGTCATCTTCACCATACCAGCATCACCAT
TCTTCAAGAACTTGGGCTCCTTCTCAATCTCCTTACCAGAACGCCTGTCAATCTTGGTCA
AGATCTCAGAGAACTTGACTGCAATGTGAGAGGTGTGGCAATCCAGGACTGGGGCGTAAC
CGTTACCAATCTGACCAGGGTGGTTCATGATGATGACCTGGGAGGTGAAGTTAGCAGCAC
CCTTGGCAGGGTCATCCTTGGAGTTGGATGCGACGTACCCTCTCTTAAGATCCTTGACAG
CAACATTCTTAACATTGAACCCAACGTTGTCACCTGGAAGTGCCTCAAGAAGAGACTCGT
GGTGCATCTCAACAGACTTGACCTCAGTGGTCAATCCTGTGGGAGCAAAGGTCACAACCA
TACCAGGCTTGATCATACCAGTCTCAACACGTCCCACTGGCACCGTTCCAATACCACCAA
TCTTGTAGACATCCTGAAGTGGGAGACGAAGGGGCTTGTCTGACGGCCTCTTGGGCTCGT
TGATCTGGTCAAGAGCCTCAAGGAGAGTTGGTCCCTTGTACCAGTCAAGGTTGGTGGACC
TCTCAATCATGTTGTCACCCTCAAATCCAGAGATGGGCACAAATGGGATTTTGTCGGGGT
TGTAACCAACCTTCTTCAAGTAGGAAGACACCTCCTTGATGATTTCATCGTACCTGGCCT
TGGAGTACTTTGGGGTAGTGGCATCCATCTACAAACAACATGTAAACAACCGCATTTAAT
GAGACAGAACACAAAAAGAGAGGCAATGTGTAGTCACATAACAGATGAAAGATATGGTTA
   
Sequence Name 66-K113987w-0022-726-D09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K113987w-0022-726-D09C-8409
TTCATTGAATTATCCTTATCATTGACTCCACCACTGGTGGTTCCCATGCTGGTATCTCCA
AGGATGGTCAGACCCGTGAGCACGCTCTACTTGCTTTCACCCTTGGTGTCAAGCAAATGA
TCTGCTGTTGTAACAAGGTAACCATATCTTTCATCTGTTATGTGACTACACATTGCTTCT
CTTTTTGTGTTCTGTCTCATTAATTGCGGTTTGTTACATGTTGTTTGTAGATGGATGCCA
CTACCCCCAAGTACTCCAAGGCCAGGTACGATGAAATCATCAAGGAGGTGTCTTCCTACT
TGAAGAAGGTTGGTTACAACCCCGACAAAATCCCATTTGTGCCCATCTCTGGATTTGAGG
GTGACAACATGATTGAGAGGTCCACCAACCTTGACTGGTACAAGGGACCAACTCTCCTTG
AGGCTCTTGACCAGATCAACGAGCCCAAGAGGCCGTCAGACAAGCCCCTTCGTCTCCCAC
TTCAGGATGTCTACAAGATTGGTGGTATTGGAACGGTGCCAGTGGGACGTGTTGAGACTG
GTATGATCAAGCCTGGTATGGTTGTGACCTTTGCTCCCACAGGATTGACCACTGAGGTCA
AGTCTGTTGAGATGCACCACGAGTCTCTTCTTGAGGCACTTCCAGGTGACAACGTTGGGT
TCAATGTTAAGAATGTTGCTGTCAAGGATCTTAAGAGAGGGTACGTCGCATCCAACTCCA
AGGATGACCCTGCCAAGGGTGCTGCTAACTTCACCTCCCAGGTCATCATCATGAACCACC
CTGGTCAGATTGGTAACGGTTACGCCCCAGTCCTGGATTGCCCCACCTCTCACATTGCAG
TCAAGTTCTCTGAGATCTTGACCAAGATTGACAAGCGTTCTGGTAAGGAGAATGAGAAGG
AGCCCA

Last Updated on 06.12.2018 16:11