Sequences from confirmation amplicon


Confirmation sequences for line 726E08 and hit At1g16920 are shown.
Line ID 726E08
Locus At1g16920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5787611
Primers used for PCR NA49 - 8474, show primer details
   
Sequence Name 07-K101562-0022-726-E08C-8409
GenBank Accession LN489210 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K101562-0022-726-E08C-8409
GCCATTTACATTGAATTTATCACAGCAAGAAGCAAGTGGAAGCTGGTGAAGATGGGAACG
CCTCTGTCCCGAAAGGTGAGAAAATCGAAGTTAAAAACGATGTCTCTGCGCTGAAGAAAC
TCGGGTGCTGCTCAAATTGAAGCCTTTTTTTGTTTTCACTGTTGAGAGAGAATTATAACC
CCCATGGTCCATTGTTTGATTATGTCCTTGTTTTGTAACACTATCGACATTTGTATGAGT
TTTAGACCATACACTACATAATGTTTATTTAACTTTATTTCAAGAATTGTTGGTGAACCA
GGCTCAATATGCATCANTTAAGCAGAGGAATCTGGATCTAAAATG
   
Sequence Name 07-K101571-0022-726-E08C-NA49
GenBank Accession LN489211 [GenBank]
Sequencing primer gene specific primer, NA49: 5'-CATTTTAGATCCAGATTCCTCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K101571-0022-726-E08C-NA49
ATTCTTGAAATAAAGTTAATAAACATTATGTAGTGTATGGTCTAAAACTCATACAAATGT
CGATAGTGTTACAAAACAAGGACATAATCAAACAATGGACCATGGGGGTTATAATTCTCT
CTCAACAGTGAAAACAAAAAAAGGCTTCAATTTGAGCAGCACCCGAGTTTCTTCAGCGCA
GAGACATCGTTTTTAACTTCGATTTTCTCACCTTTCGGGACAGAGGCGTTCCCATCTTCA
CCAGCTTCCACTTGCTTCTTGCTTGTGATAAATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAAGATTA

Last Updated on 06.12.2018 16:11