Sequences from confirmation amplicon


Confirmation sequences for line 726E10 and hit At3g60210 are shown.
Line ID 726E10
Locus At3g60210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22252615
Primers used for PCR 57jd - 8474, show primer details
   
Sequence Name 87-K113077-0022-726-E10C-57jd
GenBank Accession LN514784 [GenBank]
Sequencing primer gene specific primer, 57jd: 5'-CATTGTTGGAAAAGAAAGCTAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K113077-0022-726-E10C-57jd
CGTCGTGAGGGGGATCGATTGAAATGTAAAAAGAAGAAAGCTAAGAGACAGAACCTCAGG
AAGCACTTCAAGGCGGACAAGGACCCTGTCTGCTTGTGGTACAACCTGCAAAACCCATTG
CAACAAATAAAAATGAAATGATCTCCTGATTAAAACAAAATCACCACCAAAAATAAAAGG
AGACGAAGAAAAAAAAAACTAAAAGGTCGTACCTTTGCCGGTTCCCATTTGGTGGAAACG
GCGTTAATTCTAAAGGAATTTCTTCGAATTCCTATAGAAATCCGTAAACAAGAAAGGAAT
CAATGATGGGGAAGACACAGACAAAAGAGAAGCTAATCCAGAAAACAAAGAAACATGGAT
TAAGATTTGATTTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAAATGTCCGCA
   
Sequence Name 60-K113075-0022-726-E10C-8409
GenBank Accession LN510307 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K113075-0022-726-E10C-8409
GCATTTCATTGAATTATCCTGATAATCAAATCTTAATCCATGTTTCTTTGTTTTCTGGAT
TAGCTTCTCTTTTGTCTGTGTCTTCCCCATCATTGATTCCTTTCTTGTTTACGGATTTCT
ATAGGAATTCGAAGAAATTCCTTTAGAATTAACGCCGTTTCCACCAAATGGGAACCGGCA
AAGGTACGACCTTTTAGTTTTTTTTTTCTTCGTCTCCTTTTATTTTTGGGGGTGATTTTG
TTTTAATCAGGAGATCATTTCATTTTTATTTGTTGCAATGGGTTTTGCAGGTTGTACCAC
AAGCAGACAGGGTCCTTGTCCGCCTTGAAGTGCTTCCTGAGGTTCTGTCTCTTAGCTTTC
TTCTTTTTACATTTTCAATCGATCCCCCTCACGACAACAAGAGTATATTTGCCATTTTCC
GGTGATTCCTTAGCTTTCTTTCCCAACA

Last Updated on 06.12.2018 16:11