Sequences from confirmation amplicon


Confirmation sequences for line 726H08 and hit At4g14090 are shown.
Line ID 726H08
Locus At4g14090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8123693
Primers used for PCR CR56 - 8474, show primer details
   
Sequence Name 82-K107250-0022-726-H08C-CR56
GenBank Accession LN514199 [GenBank]
Sequencing primer gene specific primer, CR56: 5'-TAGAGTCACAAGAGCTGACGGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K107250-0022-726-H08C-CR56
TCACCGGTGGTGATCAGTGGCAGTTTCGGTAATTTAATCGGTTCAACGTCGAAGAGATGT
TTGTAAGAGGTGTTGAAGTAGTAGTAGTAGATGTCTAGTACAGTAGCTGGTTCAATCCAG
AGAAGTGTAGTTGGGAGGTGAAACTCACGCGCTACCGTAGAAACCCACGGGACGAGAACA
GAGTAGATTACCCCGGTGATAGGCTCTGTTTCGGTGGTGGCGTCAAGATTGGCTTTGATG
ATGTCTCTCAGGGCGTTTGAACCACATCGTTTGAGTTCGGACATGTAGATTTTCTGGTCT
TCGAATGACTTGAGACCGTCGTCGAATCCATCGGTGAACCAAGCGAAGGATAGACCTTTT
GTGGAAGGTGGCTCGCCCATACGTCGGTGAGCAGAGACTGCGGTGGAGTATGTGACGGTT
GCACCGTGGTGGATGAGGCGGTTGGCTAGTTGAAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCAT
TATTA
   
Sequence Name 66-K107249-0022-726-H08C-8409
GenBank Accession LN511622 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K107249-0022-726-H08C-8409
CACGGTGCACCGTCCATACTCCACCGCAGTCTCTGCTCACCGACGTATGGGCGAGCCACC
TTCCACAAAAGGTCTATCCTTCGCTTGGTTCACCGATGGATTCGACGACGGTCTCAAGTC
ATTCGAAGACCAGAAAATCTACATGTCCGAACTCAAACGATGTGGTTCAAACGCCCTGAG
AGACATCATCAAAGCCAATCTTGACGCCACCACCGAAACAGAGCCTATCACCGGGGTAAT
CTACTCTGTTCTCGTCCCGTGGGTTTCTACGGTAGCGCGTGAGTTTCACCTCCCAACTAC
ACTTCTCTGGATTGAACCAGCTACTGTACTAGACATCTACTACTACTACTTCAACACCTC
TTACAAACATCTCTTCGACGTTGAACCGATTAAATTACCGAAACTGCCACTGATCACCAC
CGGTGACCTCCCGTCGTTTCTTCAACCTTCGAAGGCATTACCGTCAGCTCTTGTGACTCT
A

Last Updated on 06.12.2018 16:11