Sequences from confirmation amplicon


Confirmation sequences for line 727D07 and hit At5g28680 are shown.
Line ID 727D07
Locus At5g28680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:10721167
Primers used for PCR 47xp - 8474, show primer details
   
Sequence Name 36-K119480-0022-727-D07C-47xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 47xp: 5'-TTAGGGTTTGGACCGGCAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K119480-0022-727-D07C-47xp
CATTAAGCTGTGAATCGTAATATTCCGGTTGACCAAATGTCGAAGGCGTCATTTGAAGCG
ATATCTCTTCCCCTCCTCCTCCGGTATTAGCATCAACATATATCGCGTAATCTTTGTAAG
TAGGGATACCTTTCCCACCTGTCCACCCGAGTATATCTGCGGGGTTTGTATCCCCTTGCG
CGGTTCTGTTGTTGATGAAAATGTTGAATACTTTCTGGTTGATTTTAGCAAGCTGGAATT
CACAGAAGTGGAGCCTCATGATATAGGTGAAATTAGTGTCAACTTGAAACATCCATGTGA
GATTTGACTTCATGTTGATATCTCCGTTTGGTCCGGGAAATGGCTGATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
   
Sequence Name 12-K119479-0022-727-D07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K119479-0022-727-D07C-8409
ATTGAATATCAGCCATTTCCCGGACCAACGGAGATATCAACATGAAGTCAAATCTCACAT
GGATGTTTCAAGTTGACACTAATTTCACCTATATCATGAGGCTCCACTTCTGTGAATTCC
AGCTTGCTAAAATCAACCAGAAAGTATTCAACATTTTCATCAACAACAGAACCGCGCAAG
GGGATACAAACCCCGCAGATATACTCGGGTGGACAGGTGGGAAAGGTATCCCTACTTACA
AAGATTACGCGATATATGTTGATGCTAATACCGGAGGAGGAGGGGAAGAGATATCGCTTC
AAATGACGCCTTCGACATTTGGTCAACCGGAATATTACGATTCACAGCTTAATGGGCTAG
AGATTTTCAAGATTGACACAATGAAGAACCTTGCCGGTCCAAACCCTA

Last Updated on 06.12.2018 16:11