Sequences from confirmation amplicon


Confirmation sequences for line 727G03 and hit At5g35210 are shown.
Line ID 727G03
Locus At5g35210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:13481733
Primers used for PCR 07sv - 8474, show primer details
   
Sequence Name 72-K116641-0022-727-G03C-07sv
GenBank Accession LN512791 [GenBank]
Sequencing primer gene specific primer, 07sv: 5'-GGAAACGTCAGAGATTAGAGGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K116641-0022-727-G03C-07sv
GAAAGCAGTTCTGGAGGTTGAGCCTGCAGTGCCGATATCTTTACTTGGTTGTTATATGCT
GAAAGATTTTGATGACAATGAAGTTTTTCTAGGGAAGATAGTGTCTTATGATACTGGGTT
ATATAGAGTGATATATGAGGATGGTGATTGTGAGGAATTGGAGAGTGGTGATCTTCGTCG
TTTAATTATTTCGGATAGTTACTTAGACGATGAGTTACGTGTTAGAAGGAAGAAATTAGA
TAAACTTATACTGAAGAAGGAGGAGAAAAAGAAACGAAATTCGCCGGAGAACAAAGCAGT
TGAGTTGCCAAATCAAGTAAATGGAGTGCAGGCACGAGCAGTTACGAACTCCGAAGATGG
TGATTCATATAGTGACTCAGAGTCGTCTGAGAGTGGAGATAAGAGAGGTTCAAGATCTGG
TTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAC
AAATGT
   
Sequence Name 59-K116640-0022-727-G03C-8409
GenBank Accession LN510087 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K116640-0022-727-G03C-8409
ATTTACATTGAATATATCCTGAACCAGATCTTGAACCTCTCTTATCTCCACTCTCAGACG
ACTCTGAGTCACTATATGAATCACCATCTTCGGAGTTCGTAACTGCTCGTGCCTGCACTC
CATTTACTTGATTTGGCAACTCAACTGCTTTGTTCTCCGGCGAATTTCGTTTCTTTTTCT
CCTCCTTCTTCAGTATAAGTTTATCTAATTTCTTCCTTCTAACACGTAACTCATCGTCTA
AGTAACTATCCGAAATAATTAAACGACGAAGATCACCACTCTCCAATTCCTCACAATCAC
CATCCTCATATATCACTCTATATAACCCAGTATCATAAGACACTATCTTCCCTAGAAAAA
CTTCATTGTCATCAAAATCTTTCAGCATATAACAACCAAGTAAAGATATCGGCACTGCAG
GCTCAACCTCCAGAACTTGCTTTTTCCCTCGATTGTTCAATTTTCGATTATCATCCTCTA
ATCCGAAACGTTTCC

Last Updated on 06.12.2018 16:11