Sequences from confirmation amplicon


Confirmation sequences for line 727G05 and hit At4g32510 are shown.
Line ID 727G05
Locus At4g32510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15686274
Primers used for PCR TF09 - 8474, show primer details
   
Sequence Name 63-K027641-022-727-G05C-8409
GenBank Accession LN510899 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K027641-022-727-G05C-8409
CCATTTCTGATATGCTATGGAGTGACATGGATACCTGTAGGAGGAATATTGTTCCCTTTG
CCATTCTTCATCCTCATTGCGTTACGACAATACATCCTCCAGAGACTTTTTGATCCATCT
CATCTCCAAGTTTTAGATTCTTCAGAGTATGAAGAGATGGTTGGTGCACCACAACGAAAC
TCCAGCTTCGGCTTCAATGGGGAATTAAGAGAGGCGCATAATATTCCACTGAGTGTTGTT
GAGAACAGTGAAGACGAGTTTTATGATGCAGAGATTCTGGATGAGATTACTACAAGCAGA
GGTGAACTCAAGCATAGAACCTTAAGTGTCAAAGAAGACA
   
Sequence Name 64-K027641-022-727-G05C-TF09
GenBank Accession LN511113 [GenBank]
Sequencing primer gene specific primer, TF09: 5'-TACCATCTGGGATCTGTCTTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K027641-022-727-G05C-TF09
CCTCTGCTTGTAGTAATCTCATCCAGAATCTCTGCATCATAAAACTCNGTCTTCACTGGT
TCTCAACAACACTCAGTGGAATATTATGCGCCTCTCTTAATTCCCCATTGAAGCCGAAGC
TGGAGTTTCGTTGTGGTGCACCAACCATCTCTTCATACTCTGAAGAATCTAAAACTTGGA
GATGAGATGGATCAAAAAGTCTCTGGAGGATGTATTGTCGTAACGCAATGAGGATGAAGA
ATGGCAAAGGGAACAATATTCCTCCTACAGGTATCCATGTCACTCCATAGCATATCAGAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11