Sequences from confirmation amplicon


Confirmation sequences for line 727H06 and hit At3g55000 are shown.
Line ID 727H06
Locus At3g55000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20383225
Primers used for PCR NF09 - 8474, show primer details
   
Sequence Name 12-K102427-0022-727-H06C-8409
GenBank Accession LN491520 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K102427-0022-727-H06C-8409
ATTTACAATTGAATATATCCTGAAGATACGTATGCTTCATTATTTTTTGATACCAAATAA
ATATGACAAGATGTTACAGATAGAAGAGCCGGGTTGTCTACATCTGGGTACAGAAAAGAT
GAATTCAACTGGAGACAAGGTAACCAAGATACACATGAAGAGGTAACAAGAGCTTCAGCG
GCGCTGGAAAATCTACAGCTTGATAGGAAAACTCGGAACTTAACATCGTCTTGGAGGTAA
GCTTTCAAGGAAGTCTCATTGAATCCCACATTGAAATGAAAACAAAGCAAGTTGTGGTAA
ATCTGATCCATCGGGCTTGCTTGTGTTGTAGGAATGTGAGGGATGGAACAAATGAAGAAG
AAGGGAGAGATTGATTTAGGCTTTGGATAAACTTTTCAGCTTCA
   
Sequence Name 12-K102428-0022-727-H06C-NF09
GenBank Accession LN491521 [GenBank]
Sequencing primer gene specific primer, NF09: 5'-GAAGCTGAAATGTTTATCCAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K102428-0022-727-H06C-NF09
TTGTTCCATCCCTCACATTCCTACACACAAGCAAGCCCGATGGATCAGATTTACCACAAC
TTGCTTTGTTTTCATTTCAATGTGGGATTCAATGAGACTTCCTTGAAAGCTTACCTCCAA
GACGATGTTAAGTTCCGAGTTTTCCTATCAAGCTGTAGATTTTCCAGCGCCGCTGAAGCT
CTTGTTACCTCTTCATGTGTATCTTGGTTACCTTGTCTCCAGTTGAATTCATCTTTTCTG
TACCCAGATGTAGACAACCCGGCTCTTCTATCTGTAACATCTTGTCATATTTATTTGGTA
TCAAAAAATAATGAAGCATACGTATCTTCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11