Sequences from confirmation amplicon


Confirmation sequences for line 728A02 and hit At4g38510 are shown.
Line ID 728A02
Locus At4g38510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18014533
Primers used for PCR SR27 - 8474, show primer details
   
Sequence Name 53-K103544-0022-728-A02C-SR27
GenBank Accession LN508124 [GenBank]
Sequencing primer gene specific primer, SR27: 5'-AGCAAAAACAAACCTCTCCTGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K103544-0022-728-A02C-SR27
ATTCCGATGTTCCCTCAATACCTGGACAAGCAAATCAAAGAAATTTACCTCGCAGTGTGT
TGAATAATAATGACTATGCAAGATTACATTGATTTACAAATGCGTTCAATTAAGCATCAA
AGTGGAGACAAGAGGAGCAGAAAACTAAAGAAGAGAGGAAGATTCAAGAAACCTGAACAA
CAGCTTTCTCTCCATCAACTTCGAGAACTTGACCACGTCGGGTAGTTCCATCCCCAAGAC
GGATATTGACAATTTCTTGGTATTTAGGCCCCTAGAGAGGAAAGCAGTATCATCAGGAAA
CCCATGGGACGAGAACATTATAAAATCAAAAATGGCTTGTCTCGTCATCCTTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACACGATTATT
   
Sequence Name 69-K103545-0022-728-A02C-8409
GenBank Accession LN512182 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K103545-0022-728-A02C-8409
CTTTACAAGGATGACGAGACAAGCCATTTTTGATTTTATAATGTTCTCGTCCCATGGGTT
TCCTGATGATACTGCTTTCCTCTCTAGGGGCCTAAATACCAAGAAATTGTCAATATCCGT
CTTGGGGATGGAACTACCCGACGTGGTCAAGTTCTCGAAGTTGATGGAGAGAAAGCTGTT
GTTCAGGTTTCTTGAATCTTCCTCTCTTCTTTAGTTTTCTGCTCCTCTTGTCTCCACTTT
GATGCTTAATTGAACGCATTTGTAAATCAATGTAATCTTGCATAGTCATTATTATTCAAC
ACACTGCGAGGTAAATTTCTTTGATTTGCTTGTCCAGGTATTTGAGGGAACATCTGGAAT
CGACAACAAGTACACAACTGTGCAATTTACAGGAGAGGTTTGTTTTTGC

Last Updated on 06.12.2018 16:11