Sequences from confirmation amplicon


Confirmation sequences for line 728C07 and hit At3g22310 are shown.
Line ID 728C07
Locus At3g22310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7889015
Primers used for PCR 09mp - 8474, show primer details
   
Sequence Name 57-K114277-0022-728-C07C-09mp
GenBank Accession LN509488 [GenBank]
Sequencing primer gene specific primer, 09mp: 5'-CAAATATTGATCTTGGCTGTAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K114277-0022-728-C07C-09mp
CTCTTATGTAATTTAGGTTGGAGATTCAGATCAGAAACTTGCAGATGGGATTACTATGTA
TTCTATCGCTGCAGATTCTTATGGAAGAGCATCCATCATTGGTCCTCTTGTAAAGGTATG
TTTTCTTTACTTTGCTTTTAAGCCAATTATCTGTATCTTGTTTTGATTCTTCATTTTTGT
TTCCAGTCGTGATTTTATAACATCTTCTCCTTTACAGGAACATGGCAAAGGAGGAAAATG
CATTGTCTTTACCCAAACAAAACGTGATGCAGATCGCCTTGCATTTGGATTGGCAAAAAG
CTATAAATGTGAAGCTTTACACGGTGACATATCTCAAGCTCAGAGAGAAAGAACACTTGC
TGGAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAA
   
Sequence Name 27-K114276-0022-728-C07C-8409
GenBank Accession LN498493 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114276-0022-728-C07C-8409
ATCCTGATTCCGCAAGTGTTCTTTCTCTCTGAGCTTGAGATATGTCACCGTGTAAAGCTT
CACATTTATAGCTTTTTGCCAATCCAAATGCAAGGCGATCTGCATCACGTTTTGTTTGGG
TAAAGACAATGCATTTTCCTCCTTTGCCATGTTCCTGTAAAGGAGAAGATGTTATAAAAT
CACGACTGGAAACAAAAATGAAGAATCAAAACAAGATACAGATAATTGGCTTAAAAGCAA
AGTAAAGAAAACATACCTTTACAAGAGGACCAATGATGGATGCTCTTCCATAAGAATCTG
CAGCGATAGAATACATAGTAATCCCATCTGCAAGTTTCTGATCTGAATCTCCAACCTAAA
TTACATAAGAGTACATGTGTCATTAAAAAATGTCACTTCATTAACTACACTGCTACAGCC

Last Updated on 06.12.2018 16:11