Sequences from confirmation amplicon


Confirmation sequences for line 728C12 and hit At3g55310 are shown.
Line ID 728C12
Locus At3g55310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20506005
Primers used for PCR 29wj - 8474, show primer details
   
Sequence Name 70-K118585-0022-728-C12C-29wj
GenBank Accession LN512467 [GenBank]
Sequencing primer gene specific primer, 29wj: 5'-AGAATCAATAAACCAATCAATCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K118585-0022-728-C12C-29wj
ATATTTTTGCTTGAAGTTTCATAGTTGTCAAAAGAATAAAAGTAAATAAATAATACTAAG
GATAACAATAAATAAAAATTATCAAACCTAAAGAATCAGCGGAGACATTGGCACGTTAAA
TTGTCTATTTGGTGATGAAGAAGAGTGTTGACTAGGATAGAAGAACCTACGGGTTGGGCA
TAAACCCGCCCTTTGTTATGTATTATATATCTAAGTTCTATTTGTACATCTTAGTTATTT
TATGGGCTTCTTCTTCTCCTCCATCGAGCACACACATAGAAAACAAACAATATGAGCAAT
CATCAAACGGTGAGATTCCTTTTGCTCACATTTCCCATCTTTATTTTTTATCTTCAAAAC
TATCAGACGATGACCATTTGAAACCCCTTGCGATGGATATGATCACTCTCCATATTACCG
TGATTTTAATCGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATT
   
Sequence Name 40-K118584-0022-728-C12C-8409
GenBank Accession LN503875 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K118584-0022-728-C12C-8409
TTTCGATTANATCACGGTAATATGGAGAGTGATCATATCCATCGCACGGTGTTTCAATGG
TCATCGTCTGATAGTTTTGAAGATAAAAAATAAAGATGGGAAATGTGAGCAAAAGGAATC
TCACCGTTTGATGATTGCTCATATTGTTTGTTTTCTATGTGTGTGCTCGATGGAGGAGAA
GAAGAAGCCCATAAAATAACTAAGATGTACAAATAGAACTTAGATATATAATACATAACA
AAGGGCGGGTTTATGCCCAACCCGTAGGTTCTTCTATCCTAGTCAACACTCTTCTTCATC
ACCAAATAGACAATTTAACGTGCCAATGTCTCCGCTGATTCTTTAGGTTTGATAATTTTT
ATTTATTGTTATCCTTAGTATTATTTATTTACTTTTATTCTTTTGACAACTATGAATACT
TCAAGCAAAAATATTATATAACCATGAGTTTCTCGCGGATTGATTGGTTATTTGATTC

Last Updated on 06.12.2018 16:11