Sequences from confirmation amplicon


Confirmation sequences for line 728F04 and hit At1g29980 are shown.
Line ID 728F04
Locus At1g29980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10503970
Primers used for PCR 06en - 8474, show primer details
   
Sequence Name 14-K110753-0022-728-F04C-06en
GenBank Accession LN492561 [GenBank]
Sequencing primer gene specific primer, 06en: 5'-TCTTGGTTTGCAACAACATTTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K110753-0022-728-F04C-06en
CGGACTCTAATTTTGAGTTCTTAACCAAACCGACAACGAGAAGAACAAAAACCCAAAACC
CAAACCCAGCTCCGATTCTCTTCGACCCGGAGAACCACACTCTAACGTCATCGATCACAG
GTCCACACAAGGAGCTCATATCATCAGTCCTCGTATTGTAATAAACACTGTAGAACGCAA
CTCTGGTTCGATCAGCCTTAGCCGTGAAATTCAAACCGGCCTTTTCGAAACTGGAGTTCG
CTTGCGCCATGTAATGAAAGTTCTGCGCCTGATCACCTGCGAATGCCATAATAGCCAATG
GTTCCTTGCATTTATCACCTGCGTGGCCTAGTGAGAAAGACAATATGTACGGCTTATCTG
CTTTGGTCTCAACCATTTGTGAAATAATTCCTTCTTTGCCTGAGAGTAGCTCGACGGCTC
GTTTGCCCTTTGGAACAGAGAAGTGATCCGAGTCAACAAACCGAACCGCTCTGTTCGATT
CAACAGTCCAACCAGGAAGAGACGAGATTTCTTCGTCGAGGTTTGTCGGAAGTAAAACAC
CAAGAGACGTGTTCCTAAACATCCATGGACCATCTTCGACATCCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 06-K110750-0022-728-F04C-8409
GenBank Accession LN488866 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K110750-0022-728-F04C-8409
TCGGACATGAGCCTTTACATTGAATTATCCTGGATGTCGAAGATGGTCCATGGATGTTTA
GGAACACGTCTCTTGGTGTTTTACTTCCGACAAACCTCGACGAAGAAATCTCGTCTCTTC
CTGGTTGGACTGTTGAATCGAACAGAGCGGTTCGGTTTGTTGACTCGGATCACTTCTCTG
TTCCAAAGGGCAAACGAGCCGTCGAGCTACTCTCAGGCAAAGAAGGAATTATTTCACAAA
TGGTTGAGACCAAAGCAGATAAGCCGTACATATTGTCTTTCTCACTAGGCCACGCAGGTG
ATAAATGCAAGGAACCATTGGCTATTATGGCATTCGCAGGTGATCAGGCGCAGAACTTTC
ATTACATGGCGCAAGCGAACTCCAGTTTCGAAAAGGCCGGTTTGAATTTCACGGCTAAGG
CTGATCGAACCAGAGTTGCGTTCTACAGTGTTTATTACAATACGAGGACTGATGATATGA
GCTCCTTGTGTGGACCTGTGATCGATGACGTTAGAGTGTGGTTCTCCGGGTCGAAGAGAA
TCGGAGCTGGGTTTGGGTTTTGGGTTTTTGTTCTTCTCGTTGTCGGTTTGGTTTAAGTAA
CTCAAAATTTAGATGTTCCGGTTTTATTCTAGTGTACCAGTAAAATGTTGTTGAAAACCA
AGA

Last Updated on 06.12.2018 16:11