Sequences from confirmation amplicon


Confirmation sequences for line 728F08 and hit At3g49120 are shown.
Line ID 728F08
Locus At3g49120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18208160
Primers used for PCR KN23 - 8474, show primer details
   
Sequence Name 37-K103252-0022-728-F08C-8409
GenBank Accession LN502580 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K103252-0022-728-F08C-8409
GCCATTTACAATTGAATATATCCTGCTTTTGTCATCAGCAGATTCAGCACACGCAATTGT
CTTAGTTAATACTCTTTTTGCTCAAAGACTAAATTTCACGTAGTTAGATGTTACTAGTTG
TTAACTGCTTTTCTTTTTCTATATTGAAAATTAATATTACTCATCGACAGTGATGTTACT
GGTTAGTTGTTAATTTCTTACACAATGATGATGCCAAAACATTTATGCAATCGGATTCAC
GAAAATAAAGTATCCGTGTTACAGTCTAAGGAATTGCATGGTGCCGTACCAAACACGATA
GAAATTTAAGATCCATCAATCATTTTTCTTTTTTGGTTGAGGGATATATATTTAAAAAAT
AATTCTAAAATCATGATTATTAATTTTTATAATTCAATTAAAATTTTGATGAATTTAAAT
ATTTTAATGTGATTATATATAAGTATTTCGGTATACAAATATTATTTCGGTATGGTTTAT
TTTCTATTGAGTTAAAATTGGTTAAAGGAGTTTCTCTATCACTTTGTTTTCATTATAATT
TAGATATTTACAGATATACCTTCACTGATTACTTGCTTCAGAGGAACA
   
Sequence Name 85-K103253-0022-728-F08C-KN23
GenBank Accession LN514536 [GenBank]
Sequencing primer gene specific primer, KN23: 5'-TTGTTCCTCTGAAGCAAGTAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K103253-0022-728-F08C-KN23
TGAAACAAAGTGATAGAGAAACTCCTTTAACCAATTTTAACTCAATAGAAAATAAACCAT
ACCGAAATAATATTTGTATACCGAAATACTTATATATAATCACATTAAAATATTTAAATT
CATCAAAATTTTAATTGAATTATAAAAATTAATAATCATGATTTTAGAATTATTTTTTAA
ATATATATCCCTCAACCAAAAAAGAAAAATGATTGATGGATCTTAAATTTCTATCGTGTT
TGGTACGGCACCATGCAATTCCTTAGACTGTAACACGGATACTTTATTTTCGTGAATCCA
ATTGCATAAATGTTTTGGCATCATCATTGTGTAAGAAATTAACAACTAACCAGTAACATC
ACTGTCGATGAGTAATATTAATTTTCAATATAGAAAAAGAAAAGCAGTTAACAACTAGTA
ACATCTAACTACGTGAAATTTAGTCTTTGAGCAAAAAGAGTATTAACTAAGACAATTGCG
TGTGCTGAATCTGCTGATGACAAAAGCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCANAAATGTAGATTTCCGCA

Last Updated on 06.12.2018 16:11