Sequences from confirmation amplicon


Confirmation sequences for line 729E09 and hit At4g02170 are shown.
Line ID 729E09
Locus At4g02170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:958586
Primers used for PCR NF07 - 8474, show primer details
   
Sequence Name 14-K102427-0022-729-E09C-OriL
GenBank Accession LN492453 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K102427-0022-729-E09C-OriL
ATTTACAATTGAATATATCCTGTAGAGACAAGTGTCTTATTTGAAATACAAACAACACAA
CGACGCCGTATTATTATAGATTTATGATGATGGTTTTATTTACTAATATGAATGAATGCA
GCTTGTTTTTGTAGATAACTGAGTCTATATATCATCTAATTACAACAACAAAAAGATGTT
TCATTTTTGATTTCATATGTACTTCATTTTCCTTTTTTTTTATGAATTTCCTTTTACCGT
ATTGTTGTTTCAAACAAATATTAATAGATAAGTAGTACATTGTTTGTTAACTGGAAGTCA
GTACCCAATTTGCCCCAAAACCTAAAATATTAGTCAAATGCAGTACGATGCAATCTTAAT
AGTTTAGGATAGGTGGGCGCAATCTAGATTGAATCATCCAATCCAATCATATATATAATT
TAATTCAAGTTTTTTTTTCAATCTTCCTACACAAAAAATAACAAATATTACCCGTTTTGG
TAATTTAATATAGAGAGTTAGGGCATCTCCAACCCTATTATATTAAATTACCGGGACGGG
TNNTNTTTGTTNTTTTTT
   
Sequence Name 14-K102428-0022-729-E09C-NF07
GenBank Accession LN492454 [GenBank]
Sequencing primer gene specific primer, NF07: 5'-AATATAATAGGGTTGGAGATGCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K102428-0022-729-E09C-NF07
AAACGGGTATATTTGTTATTTTTTGTGTAGGAAGATTGAAAAAAAAACTTGAATTAAATT
ATATATATGATTGGATTGGATGATTCAATCTAGATTGCGCCCACCTATCCTAAACTATTA
AGATTGCATCGTACTGCATTTGACTAATATTTTAGGTTTTGGGGCAAATTGGGTACTGAC
TTCCAGTTAACAAACAATGTACTACTTATCTATTAATATTTGTTTGAAACAACAATACGG
TAAAAGGAAATTCATAAAAAAAAAGGAAAATGAAGTACATATGAAATCAAAAATGAAACA
TCTTTTTGTTGTTGTAATTAGATGATATATAGACTCAGTTATCTACAAAAACAAGCTGCA
TTCATTCATATTAGTAAATAAAACCATCATCATAAATCTATAATAATACGGCGTCGTTGT
GTTGTTTGTATTTCAAATAAGACACTTGTCTCTACAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11