Sequences from confirmation amplicon


Confirmation sequences for line 729H10 and hit At4g04040 are shown.
Line ID 729H10
Locus At4g04040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1942246
Primers used for PCR 28ve - 8474, show primer details
   
Sequence Name 29-K117967-0022-729-H10C-28ve
GenBank Accession LN499467 [GenBank]
Sequencing primer gene specific primer, 28ve: 5'-AGGTCTGCTTCTATCCACCACTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117967-0022-729-H10C-28ve
ACCTCAGCAACTCGAACTGAAAACCTGTTTATTTTGAACCTTTATATGAATTTTCTCTTC
AGGTTGCTAAAATAGAGACAGAGAAAATGTTGATTCAAATGGTTGAAACCGAGCTAGAGA
AAAAGAAGACAGAGGGTACATATGAACGCGAGTTCATGGGGAAATCACACTTCTTTGGGT
AATGAATTAGCCTAAAACATTTGTTAATAACTAATTAGCGTCAACATAATCATATTCTCA
TTCTCATTGTGTTTTAGGTATGAAGGAAGATGCGGCTTACCTACAAATTTCGATGCAACT
TACTGCTACGCATTAGGTTACGGTGCTGGATCACTTCTCCAGAGCGGAAAGACCGGATTA
ATTTCATCGGTTTAGAATTTTTCTTCCTTTCTTGATAATATCACAAACTGTTTCTTTATT
TCAAGATTATTTATATTCCCCTTTGTGATGTTTTGCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 13-K117966-0022-729-H10C-8409
GenBank Accession LN492239 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117966-0022-729-H10C-8409
ATTTACATTGAAATATCCTGCAAACATCACAAAGGGGAATATAAATAATCTTGAAATAAA
GAAACAGTTTGTGATATTATCAAGAAAGGAAGAAAAATTCTAAACCGATGAAATTAATCC
GGTCTTTCCGCTCTGGAGAAGTGATCCAGCACCGTAACCTAATGCGTAGCAGTAAGTTGC
ATCGAAATTTGTAGGTAAGCCGCATCTTCCTTCATACCTAAAACACAATGAGAATGAGAA
TATGATTATGTTGACGCTAATTAGTTATTAACAAATGTTTTAGGCTAATTCATTACCCAA
AGAAGTGTGATTTCCCCATGAACTCGCGTTCATATGTACCCTCTGTCTTCTTTTTCTCTA
GCTCGGTTTCAACCATTTGAATCAACATTTTCTCTGTCTCTATTTTAGCAACCTGAAGAG
AAAATTCATATAAAGGTTCAAAATAAACAGGTTTTCAGTTCGAGTTTGCTTGAGGTTTTA
GCCTTTTAATTTGTTTGAATCAGTTAGTGGTGGAA

Last Updated on 06.12.2018 16:11