Sequences from confirmation amplicon


Confirmation sequences for line 730A09 and hit At2g18680 are shown.
Line ID 730A09
Locus At2g18680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8094975
Primers used for PCR 18vy - 8474, show primer details
   
Sequence Name 30-K118269-0022-730-A09C-18vy
GenBank Accession LN499909 [GenBank]
Sequencing primer gene specific primer, 18vy: 5'-TACCATTACCAAAAAGCAATAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118269-0022-730-A09C-18vy
TGGATCGGTTTTCGTTCTTGAATGTTATAATGGAGGTTGCGGGTATTCTAAATGAATCCC
GCAAACTCTCCTCAAGAACAAAAAGTTGATGTTCTCAGTCTTGGTATTCCCTCTCTTACT
CAATTGTTTAGTTTACTTGTTCAACGCCATTGCCATCAAACCCGAGATAACAAACTTGAT
CCTTGAATCAAGTTTGTTACCTATGACAGATCCAAACACCCCGGAATTTGCTGCCCATCT
TATGAGAGTCTTTGTAGATTTTCGCCAGTTTGTCAATTCTTTATACATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 15-K118268-0022-730-A09C-8409
GenBank Accession LN493175 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118268-0022-730-A09C-8409
ATAAAGAATTGACAAACTGGCGAAAATCTACAAAGACTCTCATAAGATGGGCAGCAAATT
CCGGGGTGTTTGGATCTGTCATAGGTAACAAACTTGATTCAAGGATCAAGTTTGTTATCT
CGGGTTTGATGGCAATGGCGTTGAACAAGTAAACTAAACAATTGAGTAAGAGAGGGAATA
CCAAGACTGAGAACATCAACTTTTTGTTCTTGAGGAGAGTTTGCGGGATTCATTTAGAAT
ACCCGCAACCTCCATTATAACATTCAAGAACGAAAACCGATCCATGGCTTTCAAAAAGAA
AATGAAAAAAAAAGCTCTTGGCTATTGCTTTTGGNTAATGGTA

Last Updated on 06.12.2018 16:11