Sequences from confirmation amplicon


Confirmation sequences for line 730B01 and hit At1g72900 are shown.
Line ID 730B01
Locus At1g72900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27433161
Primers used for PCR 14ce - 8474, show primer details
   
Sequence Name 45-K108442-0022-730-B01C-14ce
GenBank Accession LN505553 [GenBank]
Sequencing primer gene specific primer, 14ce: 5'-GAACCCACTCCAAAAATAGACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K108442-0022-730-B01C-14ce
ACTTATTAAGTTAGTTACTTAATGCATATGACTCAAGGGATCTAACTTATTAAACTGATT
CCAACACTTTCTCATAACCAGATCTTCTACTTTGAGAGCTAAAACATTCCGTGGCAAAAT
TCATTGCTTTATATATACAAAGCCCACTCGAACGCAGCAGAAATGTCGTCTCTCTTTTTA
AAGGCCAATGATCGGAAGCTACGACAAAATTTCTGGAATGTCAAAAACTCAGCTTCGTAG
ACAAGCTGTATACCATAAGAAATCAACAACTGCTTATCTTTTGTAGTGATGATAACAACA
CTCCCCGGACCAAAACCGCTGAAATCCCCTGCAAGAGCATCTAACTGTTCAAGCTTATCG
ACGTCGTCTGCCACAAGCAGAACCTTTTGCTTCTTGAGACTTTGTTTGCTAGAGTTTTCT
CCTTGAATATTATCCAGAAACTCTTTGTGTAGATGGCTTTCAAAGTAGTTACCCTGACAA
ATCCGTTTCACGTTTCCAAGTAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 33-K108441-0022-730-B01C-8409
GenBank Accession LN501027 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K108441-0022-730-B01C-8409
CATCTACTTGGAACGTGAAACGGATTTGTCAGGGTAACTACTTTGAAAGCCATCTACACA
AAGAGTTTCTGGATAATATTCAAGGAGAAAACTCTAGCAAACAAAGTCTCAAGAAGCAAA
AGGTTCTGCTTGTGGCAGACGACGTCGATAAGCTTGAACAGTTAGATGCTCTTGCAGGGG
ATTTCAGCGGTTTTGGTCCGGGGAGTGTTGTTATCATCACTACAAAAGATAAGCAGTTGT
TGATTTCTTATGGTATACAGCTTGTCTACGAAGCTGAGTTTTTGACATTCCAGAAATTTT
GTCGTAGCTTCCGATCATTGGCCTTTAAAAAGAGAGACGACATTTCTGCTGCGTTCGAGT
GGGCTTTGTATATATAAAGCAATGAATTTTGCCACGGAATGTTTTAGCTCTCAAAGTAGA
AGATCTGGTTATGAGAAAGTGTTGGAATCAGTTTAATAAGTTAGATCCCTTGAGTCATAT
GCATTAAGTTAACTAAACTTAATAAGTTGTGTGGCTTTCAAATAAGGTTGTTTTGTCTAT
TTTTGGAGTGGGT

Last Updated on 06.12.2018 16:11