Sequences from confirmation amplicon


Confirmation sequences for line 730C07 and hit At1g69430 are shown.
Line ID 730C07
Locus At1g69430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26098787
Primers used for PCR 41cy - 8474, show primer details
   
Sequence Name 31-K109234-0022-730-C07C-41cy
GenBank Accession LN500200 [GenBank]
Sequencing primer gene specific primer, 41cy: 5'-CAACTCTTAAAGCAGGGACAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K109234-0022-730-C07C-41cy
GAGCTTCAAGCATCACAGCTTCCATACTATAAGACCGGCAACTAAAATAGAACACTGCAC
TCATCATTGTATCAATAAGCACCACAAATGAATACATCACCACAAGTAGTGGACCTTCCC
ATAACCTCGAAGAACCATCTCCATAACTCAAACTCTTCACTCTATGCTCAAACAATCCTT
CCACAAACGTCAACCCTATTGTTGATCCTAGAAATATCAACAAACCAACCTGAGTCTGTC
CTTTGATCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 15-K109233-0022-730-C07C-8409
GenBank Accession LN493011 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K109233-0022-730-C07C-8409
TTTCATTGAATATTGATCAAAGGACAGACTCAGGTTGGTTTGTTGATATTTCTAGGATCA
ACAATAGGGTTGACGTTTGTGGAAGGATTGTTTGAGCATAGAGTGAAGAGTTTGAGTTAT
GGAGATGGTTCTTCGAGGTTATGGGAAGGTCCACTACTTGTGGTGATGTATTCATTTGTG
GTGCTTATTGATACAATGATGAGTGCAGTGTTCTATTTTAGTTGCCGGTCTTATAGTATG
GAAGCTGTTGAAGCTTTGGAAGCTTCTGGAGAGATTGTGTCCCAAGATTTGTCCCTGCTT
TAAGAGTTG

Last Updated on 06.12.2018 16:11