Sequences from confirmation amplicon


Confirmation sequences for line 730G10 and hit At5g27410 are shown.
Line ID 730G10
Locus At5g27410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9682460
Primers used for PCR 31fr - 8474, show primer details
   
Sequence Name 45-K111470-0022-730-G10C-31fr
GenBank Accession LN505569 [GenBank]
Sequencing primer gene specific primer, 31fr: 5'-AAAAGTCAAACAAGAGAGCCTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K111470-0022-730-G10C-31fr
ACCCACAGGCTACACAGGTCAACAGCATAATATGAGAGAGTTTGAGAGCTAAAACANGCA
CGCACTTTCTATTCAAAAACGTAAAACACCACANGCTTTCCTCTGAAATACGTGCCGTTT
TCCTCGTATGCAATGTTTTTTTCCTGCAATCACAAAACCTTTTCAGTACTTCAAAAATAA
AAAACCAATGATGAAATTATGTTAACGTTGATTTCATTTTTCCAAAATAAGGTTCCTTTT
GTTCAACGTTCTTGTTAGAGTTTACGAATCTAGATGAACACATTTAACAAGCCTGGTTTA
CCATTCGTGTTCTACGATCAAACACACGCCCGACGTAAATATCATAAAATGTATTCAATT
GATAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 29-K111469-0022-730-G10C-8409
GenBank Accession LN499347 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K111469-0022-730-G10C-8409
CTATCATTGAATAATTTTATGATATTTACGTCGGGCGTGTGTTTGATCGTAGAACACGAA
TGGTAAACCAGGCTTGTTAAATGTGTTCATCTAGATTCGTAAACTCTAACAAGAACGTTG
AACAAAAGGAACCTTATTTTGGAAAAATGAAATCAACGTTAACATAATTTCATCATTGGT
TTTTTATTTTTGAAGTACTGAAAAGGTTTTGTGATTGCAGGAAAAAAACATTGCATACGA
GGAAAACGGCACGTATTTCAGAGGAAAGCTGTGGTGTTTTACGTTTTTGAATAGAAAGTG
CGTGCTGTTTTAGCTCTCAAACTCTCTCATATTATGCTGTTGACCTGTTGTAGCCTGTGG
GTCAATGTAATCACAGTTGGTCGCAAATCCAAGTCAAGTCAGGCTCTCTTGTTTGACTTT

Last Updated on 06.12.2018 16:11