Sequences from confirmation amplicon


Confirmation sequences for line 730H12 and hit At3g27329 are shown.
Line ID 730H12
Locus At3g27329 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10114546
Primers used for PCR 48qp - 8474, show primer details
   
Sequence Name 73-K115691-0022-730-H12C-48qp
GenBank Accession LN512956 [GenBank]
Sequencing primer gene specific primer, 48qp: 5'-AGCACACCTTGAAGTTGTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K115691-0022-730-H12C-48qp
TAAACCTAATTGAGTCGTACAAGAGATTATGTAACAAGGCAACGCCTTTTATAAGATATA
CAAGTTAAACCTAATCGAGTTGTACAAGAGATTATGTAACAAGGCAACGCCTTTTATAAG
ATATACTAGTTAAACCTAATTGATTTGTACAAGAGATTGATTAATTGGGCTAAGGGGTCT
CATTGTCCAAGAGCTATATAACTGTTTTGTATGGTTGTCTTCAATTGGTGGGGACATATT
ATATGGTGATTCTTCTTATGGTGATGGGTCTGGTGAGATCGAATGATTAAAGATACATTC
CATAGATAAAAGCATCAGTCCAAGGCTCATCACCAATTGACAATTTTTATGTTTTTCTTT
GCCAAATGATAATACTTGAGATCCAAATTTATCAATATATATATTTTTTCTTACATTTAA
ATCATAATATAATTTGAGAAGATTTTTTTCTCATACATGTATAATTTCTTTTGTTTTTAA
ATCATAAGAAAATTCTTCTTTTTCATTTTTAGATAGCTTGTCAATAATTTTAATAAATCC
AATCTACATGGCATTAGCAATGATATATTCAAACCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 25-K115690-0022-730-H12C-8409
GenBank Accession LN497594 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115690-0022-730-H12C-8409
TATCATTGCTAATGCCATGTAGATTGGATTTATTAAAATTATTGACAAGCTATCTAAAAA
TGAAAAAGAAGAATTTTCTTATGATTTAAAAACAAAAGAAATTATACATGTATGAGAAAA
AAATCTTCTCAAATTATATTATGATTTAAATGTAAGAAAAAATATATATATTGATAAATT
TGGATCTCAAGTATTATCATTTGGCAAAGAAAAACATAAAAATTGTCAATTGGTGATGAG
CCTTGGACTGATGCTTTTATCTATGGAATGTATCTTTAATCATTCGATCTCACCAGACCC
ATCACCATAAGAAGAATCACCATATAATATGTCCCCACCAATTGAAGACAACCATACAAA
ACAGTTATATAGCTCTTGGACAATGAGACCCCTTAGCCCAATTAATCAATCTCTTGTACA
AATCAATTAGGTTTAACTAGTATATCTTATAAAAGGCATTGCCTTGTTACATAATCTCTT
GTACAACTCAATTAGGTTTAACTTGTATATCTTATAAAAGGCATTGCCTTGTTACATAAT
CTCTTGTACAACTCAATTAGGTTTAACTTGTATATCTNATATAAGGCATTGCCTTGTTAC
ATAATAAACTAAGAAATATATTTTACAAC

Last Updated on 06.12.2018 16:11