Sequences from confirmation amplicon


Confirmation sequences for line 731C12 and hit At2g34510 are shown.
Line ID 731C12
Locus At2g34510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14545481
Primers used for PCR 07en - 8474, show primer details
   
Sequence Name 28-K116610-0022-731-C12C-07en
GenBank Accession LN499005 [GenBank]
Sequencing primer gene specific primer, 07en: 5'-TAGATAGAGCCTTTCTCCACCGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116610-0022-731-C12C-07en
TCTACAGCGTGACGGCCCTCAGGGCACGATCAAGATCATCCCGCCTCCTTTTTGACCGGA
CTTGATTAGCTCCACCGTACCATCGGATCGCCAGCTCGGGATCTCGGAGGTGTCCTCGAT
GATTGCGTCATCAGGGAAGCCGTTTGACGGCGGTGTCTCAAAGTCGCCGTTAACCACCAA
GCCTTGAAAAAAGATTCCGGCAGAACAAGAAAGTTAGTTGTAATTTATTGAGAAAAAGGG
AATAATTACGGGATTTAAGAAAATGGTGTCAGAAAATAAAAATACTAGGATGGAGACGTA
TAACAATTAGTTGGTCTCTTAATAACAACAATAGATCTTTCATCTTTTACGTCCATTTTT
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCACGGGTTATTATAAT
   
Sequence Name 12-K116609-0022-731-C12C-8409
GenBank Accession LN491750 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116609-0022-731-C12C-8409
CATTTCAATTGAATATATCCTGAAAAATGGACGTAAAAGATGAAAGATCTATTGTTGTTA
TTAAGAGACCAACTAATTGTTATACGTCTCCATCCTAGTATTTTTATTTTCTGACACCAT
TTTCTTAAATCCCGTAATTATTCCCTTTTTCTCAATAAATTACAACTAACTTTCTTGTTC
TGCCGGAATCTTTTTTCAAGGCTTGGTGGTTAACGGCGACTTTGAGACACCGCCGTCAAA
CGGCTTCCCTGATGACGCAATCATCGAGGACACCTCCGAGATCCCGAGCTGGCGATCCGA
TGGTACGGTGGAGCTAATCAAGTCCGGTCAAAAACAAGGCGGGATGATCTTGATCGTGCC
TGAGGGCCGTCACGCTGTTAGATTAGGAAACGATGCAGAGATCAGCCAAGAACTTACGGT
GGAGAAAGCNTCTATCTA

Last Updated on 06.12.2018 16:11