Sequences from confirmation amplicon


Confirmation sequences for line 731D06 and hit At5g18990 are shown.
Line ID 731D06
Locus At5g18990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6341060
Primers used for PCR 54hw - 8474, show primer details
   
Sequence Name 57-K112689-0022-731-D06C-54hw
GenBank Accession LN509467 [GenBank]
Sequencing primer gene specific primer, 54hw: 5'-CTTTTTACTCGGTAGGATTTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K112689-0022-731-D06C-54hw
GAGGCCGACCTACTTCCTAGATGCACCATCCAAGGCGCGGTTGATTTCATCTTAGGGAGC
GGCCAATCTATTTACCAGGTTACATTCTCCACAAAATGATATTGGTTTTATATACTATAA
TAACATCATACGAATACCAAACATAAATTAATTCTAAAACATTTGAAGATTTGCCTTTAG
ACTGGCTAAGAATTTTATTACATTGTCTATGATTGGAACCTTTTCTATGGAAAGTATAGA
TATGATCAAAAATTTAATCCAAACTAATAATGGCTTCATGTCCGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 25-K112688-0022-731-D06C-8409
GenBank Accession LN497519 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K112688-0022-731-D06C-8409
ATTGATATATCCGGACATGAAGCCATTATTAGTTTGGATTAAATTTTTGATCATATCTAT
ACTTTCCATAGAAAAGGTTCCAATCATAGACAATGTAATAAAATTCTTAGCCAGTCTAAA
GGCAAATCTTCAAATGTTTTAGAATTAATTTATGTTTGGTATTCGTATGATGTTATTATA
GTATATAAAACCAATATCATTTTGTGGAGAATGTAACCTGGTAAATAGATTGGCCGCTCC
CTAAGATGAAATCAACCGCGCCTTGGATGGTGCATCTATGGAAGTAGTGTCGGCCATCCG
AGTCCCACAAGGTATCTTGAATTCCAGCAAATCCTACCAGGTAAAAA

Last Updated on 06.12.2018 16:11