Sequences from confirmation amplicon


Confirmation sequences for line 731D07 and hit At1g24570 are shown.
Line ID 731D07
Locus At1g24570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8707844
Primers used for PCR 22sr - 8474, show primer details
   
Sequence Name 72-K116549-0022-731-D07C-22sr
GenBank Accession LN512790 [GenBank]
Sequencing primer gene specific primer, 22sr: 5'-AGGTGACAAAGCAAATTGAGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K116549-0022-731-D07C-22sr
CTCAGAAATTAATTACAGTTTGTTTTATCGATAAACAGGAGTGAGATGGTGTTGATGACA
ATTGGAGCTTATAATCAAAAGTTGTAATAAAAACCTAACTATGGGAATCTTGTCACGCAG
GTAAAAAAGACCCTTTGTCTCGTTTCTCTTATTGTTCTCTCTAAAATCAGATTGGTGATG
ATTGTTGTTATAACAGCCCAATTAGTAGTAGGAGATCATCAGATGATAGCATGAAGCTTT
TTACAACAGCATTGTTTGCTATAGCTTTTGGCATTGTTATTGGACTATCTTTTCCATCTT
TATGGATCACTGAGGTTCTTCTTATTTCTCTTCAAATCTTTTACTTCTTAGATTTTATAC
TTCGTTTGTCGCATGCCATTTTTGTATTGTGTAGACAAGTCTCCCGAGAAACCTTCTTCG
CTCCATTGCTATCTCACTTAGAGATTCCGGAATTGCAACTCCTCACAAAGCCATTGATAT
CTCAAAGGCATGTTTTATGTAGGAATCTACACATCTTTTTCATGTTTTAAGCACAAAAAC
CAAAGTTTAAGGCATGTTTTGGTGCAGATATGGGAACCATCAAATCCTCGAGGCGCTGAG
AGATTACCATCCGGTATCATTGCATCCGAATCGGACTTGTACTTGCGCAGATTATGGGGG
AACCCTGATGAGGTTTTTCCTCACTCTTTTGATATGACATCTTTCATATGTGATCAATGA
ACATTAGCTTCAATTTTATTTTCTCTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGT
   
Sequence Name 32-K116548-0022-731-D07C-8409
GenBank Accession LN500729 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K116548-0022-731-D07C-8409
ATTACAGAGAAAAAAATTGAAGCTAATGTTCATTGATCACATATGAAAGATGTCATATCA
AAAGAGTGAGGAAAAACCTCATCAGGGTTCCCCCATAATCTGCGCAAGTACAAGTCCGAT
TCGGATGCAATGATACCGGATGGTAATCTCTCAGCGCCTCGAGGATTTGATGGTTCCCAT
ATCTGCACCAAAACATGCCTTAAACTTTGGTTTTTGTGCTTAAAACATGAAAAAGATGTG
TAGATTCCTACATAAAACATGCCTTTGAGATATCAATGGCTTTGTGAGGAGTTGCAATTC
CGGAATCTCTAAGTGAGATAGCAATGGAGCGAAGAAGGTTTCTCGGGAGACTTGTCTACA
CAATACAAAAATGGCATGCGACAAACGAAGTATAAAATCTAAGAAGTAAAAGATTTGAAG
AGAAATAAGAAGAACCTCAGTGATCCATAAAGATGGAAAAGATAGTCCAATAACAATGCC
AAAAGCTATAGCAAACAATGCTGTTGTAAAAAGCTTCATGCTATCATCTGATGATCTCCT
ACTACTAATTGGGCTGTTATAACAACAATCATCACCAATCTGATTTTAGAGAGAACAATA
AGAGAAACGAGACAAAGGGTCTTTTTTACCTGCGTGACAAGATTCCCATAGTTAGGTTTT
TATTACAACTTTTGATTATAAGCTCCAATTGTCATCAACACCATCTCACTCCTGTTTATC
GATAAAACAAACTGTAATTAATTTCTTGAGCTATACGATCAAAACAAAGCACGAATGATC
TGATTAAAAGACTCA

Last Updated on 06.12.2018 16:11