Sequences from confirmation amplicon


Confirmation sequences for line 731E02 and hit At1g70340 are shown.
Line ID 731E02
Locus At1g70340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26505142
Primers used for PCR 29ve - 8474, show primer details
   
Sequence Name 89-K117739-0022-731-E02C-29ve
GenBank Accession LN514982 [GenBank]
Sequencing primer gene specific primer, 29ve: 5'-TCTCCTTTGCTAACCTAATCGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K117739-0022-731-E02C-29ve
TTTGATCTCACTGTCTTCCCTTCGCTCTGCTGCTTCAGGACAATCCGTCTCTTTGATCTC
CTTTTCTCCTGATCAATAGAATTATAAGCGATTTCATGCAGGATCGAAGAAGACCCTTCT
TCTGTTGGTTTCTGATTTTCGGTTCTTGCGTTTATGTTCTGTGACTTGTTCTCAGCATCT
GGAGTAGCAGCTACTTCAATGGAAGTGAGTTCGCCAATGGTTTGTGCCATCTGCTGGTGA
AACTCCATGAACTTCTCAAAGCACACCACAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 73-K117738-0022-731-E02C-8409
GenBank Accession LN512966 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K117738-0022-731-E02C-8409
ATTTACAATTGAATATATCCTGTGGTGTGCTTTGAGAAGTTCATGGAGTTTCACCAGCAG
ATGGCACAAACCATTGGCGAACTCACTTCCATTGAAGTAGCTGCTACTCCAGATGCTGAG
AACAAGTCACAGAACATAAACGCAAGAACCGAAAATCAGAAACCAACAGAAGAAGGGTCT
TCTTCGATCCTGCATGAAATCGCTTATAATTCTATTGATCAGGAGAAAAGGAGATCAAAG
AGACGGATTGTCCTGAAGCAGCAGAGCGAAGGGAAGACAGTGAGATCAAATGATGAGAAC
AAGAACCCAGCTTCTGGCGGTATAAGCAACACGATTAGGTTAGCAAAGGAGA

Last Updated on 06.12.2018 16:11