Sequences from confirmation amplicon


Confirmation sequences for line 731G08 and hit At2g45030 are shown.
Line ID 731G08
Locus At2g45030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18575848
Primers used for PCR 45uv - 8474, show primer details
   
Sequence Name 88-K117671-0022-731-G08C-45uv
GenBank Accession LN514894 [GenBank]
Sequencing primer gene specific primer, 45uv: 5'-TCCTTAGCTGTTCAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K117671-0022-731-G08C-45uv
CTTTGAGCTAAACGCAACATATTTTTCCTTCAATTCTGGTTTTAAACATTTGCAACTGAA
ATCTATTTTTTGGTGTTAGTTCTCGAAAGCATTGAATCGATTCCAGAAAGAGGATCCTAC
TTTCCGTGTGGGTTTGGATCCCGAGAGTGGCCAGGTATCATCTAAAAACTCTTTTGCTTC
TCAGCACCTCTCTCTTAATTCACCCCATAGTATTTAACATTGATGATTCTTCCATTTATC
CTTCATGGGACAGACGATTATTTCTGGTATGGGGGAATTGCATTTGGACATCTATGTTGA
ACGCATGCGAAGAGAATATAAGGTGCCTGCCTGACTTTAAACCTTCCCAATGTTCTCACT
CTATTGCTTCTCGACAAAACCCTTATTTCTGCTTATTTTTGGAAATATCAAATAGGTTGA
TGCGACTGTGGGCAAACCCCGTGTCAATTTTAGGGAGACTATTACTCAGCGGGCAGAGTT
TGATTATTTACATAAGAAGCAAAGTGGAGGAGCAGGTCAATACGGTAGAGTTACAGGGTG
AGTATCAAAAACTTATAGACAATGATATATTCAATTGATATATTATTTACAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCA
   
Sequence Name 40-K117670-0022-731-G08C-8409
GenBank Accession LN503854 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117670-0022-731-G08C-8409
ATTGAATATTCCTGTAAATAATATATCAATTGAATATATCATTGTCTATAAGTTTTTGAT
ACTCACCCTGTAACTCTACCGTATTGACCTGCTCCTCCACTTTGCTTCTTATGTAAATAA
TCAAACTCTGCCCGCTGAGTAATAGTCTCCCTAAAATTGACACGGGGTTTGCCCACAGTC
GCATCAACCTATTTGATATTTCCAAAAATAAGCAGAAATAAGGGTTTTGTCGAGAAGCAA
TAGAGTGAGAACATTGGGAAGGTTTAAAGTCAGGCAGGCACCTTATATTCTCTTCGCATG
CGTTCAACATAGATGTCCAAATGCAATTCCCCCATACCAGAAATAATCGTCTGTCCCATG
AAGGATAAATGGAAGAATCATCAATGTTAAATACTATGGGGTGAATTAAGAGAGAGGTGC
TGAGAAGCAAAAGAGTTTTTAGATGATACCTGGCCACTCTCGGGATCCAAACCCACACGG
AAAGTAGGATCCTCTTTCTGGAATCGATTCAATGCTTTCGAGAACTAACACCAAAAAATA
GATTTCAGTTGCAAATGTTTAAAACCAGAATTGAAGGAAAAATATGTTGCTGTTTTAGCT
TCAAAGTTCACAGAAGTTACCTGTCCTCCAGAATCTTTTGAAACTGGTTG

Last Updated on 06.12.2018 16:11