Sequences from confirmation amplicon


Confirmation sequences for line 732A11 and hit At1g56710 are shown.
Line ID 732A11
Locus At1g56710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21258738
Primers used for PCR NF06 - 8474, show primer details
   
Sequence Name 15-K102427-0022-732-A11C-8409
GenBank Accession LN492919 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K102427-0022-732-A11C-8409
TTTACAATTGAATATTATCAATTACATTAAATCTACATGGTTGAGTTGTGTCACTCATGG
TATGTTATCAGAAACAGAGCCTTAAAGGCTGGGAATCTAAAGCCTCCTCTATAAGAGAGG
CGCATAGAGTTTGGCAGATGGAAATATTAATGCAAGTTATGAATGTGATACCTAATCCCA
TGGCTTGGACCGCACGTAACATTCTTAATATCGACATTAAAGCAGCCGGCTCCGATAGAA
ATACAATCATCTCCTGTTATAACCAAATAAGTAACATAAAACTGATTGGAATCAATGAAA
TTTTACTATTAAGCAACTGCGAAAACGGGAAAACATACCGTTGGAAATCATGGAATTGCG
AATCTGAACATTGTGTGTATTCTCAATATGGATCCCATCAGTGTTAGGACTAGAACCTGG
TGCCTTGATTATCACGGAATCAACCACGACGTCACTGCAGTTGTCAAACCTTACATGAA
   
Sequence Name 15-K102428-0022-732-A11C-NF06
GenBank Accession LN492920 [GenBank]
Sequencing primer gene specific primer, NF06: 5'-TTTCATGTAAGGTTTGACAACTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K102428-0022-732-A11C-NF06
TCAAGCACCGGTTCTAGTCCTAACACTGATGGGATCCATATTGAGAATACACACAATGTT
CAGATTCGCAATTCCATGATTTCCAACGGTATGTTTTCCCGTTTTCGCAGTTGCTTAATA
GTAAAATTTCATTGATTCCAATCAGTTTTATGTTACTTATTTGGTTATAACAGGAGATGA
TTGTATTTCTATCGGAGCCGGCTGCTTTAATGTCGATATTAAGAATGTTACGTGCGGTCC
AAGCCATGGGATTAGGTATCACATTCATAACTTGCATTAATATTTCCATCTGCCAAACTC
TATGCGCCTCTCTTATAGAGGAGGCTTTAGATTCCCAGCCTTTAAGGCTCTGTTTCTGAT
AACATACCATGAGTGACACAACTCAACCATGTAGATTTAATGTAATTGATAATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATTCA

Last Updated on 06.12.2018 16:11